Springer, Heidelberg, 2011, 244 pages
In the last decade, fluorescence microscopy has evolved from a classical retrospective microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
Fundamentals of Optical Microscopy
The White Confocal: Continuous Spectral Tuning in Excitation and Emission
Second/Third Harmonic Generation Microscopy
Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles
New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy
Improving Image Formation by Pushing the Signal-to-Noise Ratio
Site-Specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes
Imaging Molecular Physiology in Cells Using FRET-Based Fluorescent Nanosensors
Measuring Molecular Dynamics by FRAP, FCS, and SPT
In Vitro–In Vivo Fluctuation Spectroscopies
Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking
Low Concentration Protein Detection Using Novel SERS Devices
Near Infrared Three-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation
A Correlative Microscopy: A Combination of Light and Electron Microscopy
In the last decade, fluorescence microscopy has evolved from a classical retrospective microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
Fundamentals of Optical Microscopy
The White Confocal: Continuous Spectral Tuning in Excitation and Emission
Second/Third Harmonic Generation Microscopy
Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles
New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy
Improving Image Formation by Pushing the Signal-to-Noise Ratio
Site-Specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes
Imaging Molecular Physiology in Cells Using FRET-Based Fluorescent Nanosensors
Measuring Molecular Dynamics by FRAP, FCS, and SPT
In Vitro–In Vivo Fluctuation Spectroscopies
Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking
Low Concentration Protein Detection Using Novel SERS Devices
Near Infrared Three-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation
A Correlative Microscopy: A Combination of Light and Electron Microscopy