Hermanson G. Bioconjugate Techniques, Second Edition

1170 Index

Iodoacetate , 120

for blocking sulfhydryls , 161

general description and use ,

109

modiﬁ cation of

amino-dextran with , 954

PE , 872

relative reactivity of , 110 ,

183

Iodoacetic anhydride

modiﬁ cation of PE , 899

Iodoacetyl , 325 , 406 , 411 , 434 ,

449 , 834 , 871

derivatives of ﬂ uorescein ,

406

dextran modiﬁ ed , 960

in biotinylation reagents ,

520

light instability of , 289

modiﬁ ed PE , 897

of

ASIB

, 325

benzophenone-4-

iodoacetamide, 328

BODIPY 530/550 IA , 451

BODIPY FL IA , 449

DCIA , 436

iodoacetyl-LC-biotin , 524

Lucifer Yellow

iodoacetamide, 458

NPIA , 296

SIAB , 288 , 847

SIAC , 295

SIAX , 293

tetramethylrhodamine-5-

(and 6)-iodoacetamide ,

425

use in immunotoxin conjuga-

tion, 847

N -Iodoacetyl- N -biotinylhexy-

lenediamine, see Iodoacetyl-

LC-biotin

Iodoacetyl group

of ICAT reagents , 651

Iodoacetyl-LC-biotin

general description and use ,

524

protein modiﬁ cation protocol ,

525

Iodobeads , 1020

general description and use ,

550

N -Iodobenzenesulfonamide , 551

IODO-BEADS , 262 , 819

IODO-GEN , 262 , 308 , 819

for iodination of SASD , 308

Iododination

using Iodo-beads , 550

N -(  -Iodoethyl) triﬂ uoroaceta-

mide, see Aminoethyl

Iodogen , 548

general description and use ,

553

iodination protocol , 555

Ion-exchange chromatography ,

996

Iron , 910 , 965

Iron complexes , 503

Irvin , 829

Isaacs , 427 , 634

Ischiropoulos , 23

Ishi , 521

Ishikawa , 283 , 284 , 807 , 850 ,

896

Ishmael , 1007 , 1025

Islam , 364 , 371

Ismaili , 329

Isobaric tag , 659

design of , 660

Isocyanate

chemistry of , 170

derived from acetyl azide

group, 454

reaction with hydroxyls , 199

silane coupling agent , 579

Isocyanate group

of ICPTES , 579

Isocyanatopropyltriethoxysilane ,

see ICPTES

Isoelectric focusing ,

117

Isoleucine , 5

solvent exposed area , 30

Isomaltose , 857

as repeating unit of dextran ,

951

Isopropanol , 885

Isothiocyanate , 41

chemistry of , 170

labeling of N-terminal amino

acids, 170

of

DTTA , 502

FITC , 401 , 403 , 915

PDITC , 996

TRITC , 416

N -( p -Isothiocyanatoben-

zyl)-diethylenetriamine-

N , N  N    , N  

-tetraacetic

acid, see DTTA

Isotope coded afﬁ nity tags

, see

ICAT

Isotope Tags , 649

Isourea linkages , 170

Ito , 194

iTRAQ , 662

Iwai , 292

Iyer , 62

Izzo , 509

J chain , 785

Jablonski , 994

Jackson , 924

Jacobsen , 293

Jacobson , 1026

Jaffe , 394

Jagannath , 905

Jaiswal , 490

Janes , 908

Janeway , 746

Jang , 519 , 735

Jansen , 353

Jansons , 889

Jay , 219

Jayabaskaran , 101

Jeanloz , 44

Jeanson , 780

Jeffamine , 115 , 125 , 976

Jeffrey , 41

Jellum , 80 , 97

Jemmerson , 930

Jennings , 219

Jentoft , 46

Jeon , 530

Jeong , 1004

Ji , 176 , 251 , 262 , 265 , 305 , 310 ,

314

Jobbagy , 170 , 402 ,

403

Johansson , 938

Johnson , 184 , 699 , 964

Index 1171

Johnston , 256

Joiris , 498 , 503

Jones ,92 , 156 , 421 , 430 , 594 ,

602

Joralemon , 351

José-Yacamán , 639

Joshi , 239

Jue , 68 , 69

Juliano , 381

Jung , 112 , 241 , 316

Kaetsu , 617

Kagedal , 814

Kakkad , 240 , 243

Kalkhof , 1009

Kallin , 155

Kalsbeek , 813

Kam , 643

Kan , 420 , 421

Kanamycins , 709

Kanan , 563

Kandel , 753

Kang , 319

Kaplan , 139 , 253 , 601 , 602

Kappa chains , 785

Kareva , 260

Karlin , 342 , 1028

Karlsson , 467

Karsten , 147

Kasermann , 628

Kasina , 498

Kassis , 430

Kato , 964

Katti

, 181 , 342

Katz , 559

Kawa

, 347

Kawamura , 818

Kawamura, Jr. , 398 , 401

Kawaoi , 131 , 800

Kay , 256 , 962

Keana , 205 , 303

Kearney , 292

Keen , 814 , 837

Keighley , 546

Keller , 56 , 531 , 971 , 977 , 996

Kellogg , 419 , 421

Kempf , 628

Kenny , 69

Kensinger , 371

Kent , 251 , 699 , 701

Kenworthy ,

707

Keresztes-Nagy , 103

Ketal , 150

Ketene , 207

Ketene amide intermediate

of diazopyruvate photolysis ,

322

Ketoamine , 147 , 173

Ketoketenimine , 228

Ketones

chemistry of , 173

coupling by reductive

amination , 231

reaction with hydrazides , 271

reactions of , 200

Ketose , 37

Kettle ,23

Keyhole limpet hemocyanin , see

KLH

Keyhole limpets , 748

Khalfan , 431

Khan , 531

Khanna , 404

Khatiwala , 311

Kho , 694

Khorana , 978

Kidambi , 709

Kiehm , 176 , 251 , 305

Kiessling , 366

Kihlberg , 147

Kiick , 684 , 692

Kim , 23 , 25 , 240 , 351 , 863 , 963 ,

1032

Kimura , 932

King

, 69 , 71 , 166 , 186 , 509

Kinsella , 108 , 159

Kiraly , 170 , 402

Kirley , 95

, 121

Kirschner , 421

Kitagawa , 287

Kiyama , 995

Klamerth , 262

Klapper , 108 , 159

Kleene , 1026

Kleppe , 164

KLH , 753 , 762 , 771

conjugation with tyrosyl-lysine

using EDC , 757

functional groups , 749

precipitation of , 758

solubility

in presence of DMSO , 749

of , 749

use as a carrier , 748

Klibanov , 938

Klotz , 81 , 103 , 108 , 159

Knauf , 937

Knoller , 241 , 243

Knowles , 208 , 263

Knutson , 400

Kobayashi , 383 , 384 , 952

Koch , 206

Kochetkov amination , 155

Kodadek , 27 , 1037 , 1039

Koganty , 147

Kohn , 268

, 269 , 275 , 612

Köhn , 693 , 697

Kojola , 476

Kolb , 680

Konigsberg ,90 , 256 , 258

Koning , 136

Konishi

, 334

Konno , 407 , 409

Kopecek , 936

Kordás , 640

Korn , 779 , 862

Kornblatt , 260

Kornﬁ eld , 20 , 46

Kornilova , 708

Koshland , 176 , 195 , 215 ,

218 ,

219 , 223

Kota , 1016

Kotite , 219

Kovacic , 256 , 258

Kozlov , 143 , 674

Kozulic , 271

Kraehenbuhl , 132

Kricka , 59 ,

380 , 971

Krieg , 312

Kronick , 461

Kroto , 627

Kruszynski , 708

Kukowska-Latallo , 364 , 372

Kulin , 598

Kull , 243

Kumakura , 617

Kumar , 207 , 971 , 984

Kung , 882

Kurstak , 802

1172 Index

Kurz , 630 , 646

Kurzchalia , 514 , 1007 , 1037

Kuwata , 101

Kwong , 263

LAB , 905 , 908

Labbe , 256

L’abbé , 681

Label transfer reagents , 306, 1020

Laburthe , 240

Lacey , 531

 -Lactalbumin , 253

Lactate dehydrogenase , 255

Lactic dehydrogenase , 335

Lactone , 367

Lactoperoxidase

immobilized , 556

in iodination of proteins , 556

Lactose ,45

designed into crosslinker , 832

lactone derivative , 367

modiﬁ cation with , 149

cystamine , 832

plant toxin binding of , 827

Lactose synthetase , 253

Lactosylamine , 149

Lactosylceramide , 871 , 878

Laemmli ,92

Lake , 260

Lakowicz , 400 , 477

Lambda chains , 785

Lambert ,69 , 281 , 829 , 830 ,

837 , 840 , 847 , 850

Lamture , 482

Landau , 584 , 925

Lane

, 746 , 784 , 819

Lange , 161

Langer , 970 , 985

Langone , 559

Lanier , 453

Lanteigne , 499

Lanthanide , 501

in ECAT reagent , 657

Lanthanide binding tag (LBT) ,

485

Lanthanide chelate

carbostyril-based , 484

containing psoralen , 209

emission properties , 476

ﬂ uorescence lifetime , 474

FRET characteristics , 477

general description and use ,

474

quenching properties , 477

Lanthanide luminescence

pyridine enhancers , 481

Lapidot

, 886

LaRochelle , 512

Larsson , 224

Lateef , 288

Lateral ﬂ ow tests , 583

Latex particles , 583 , 882

Latham , 1032

Latham-Timmons , 669

Laumonnier , 1026

Launay , 387

Lavender oil , 430

LC-SPDP , 76 , 279 , 280

activation of

liposomes , 876

PE , 874

in conjugation of phyco-

biliproteins, 464

for immunotoxin preparation ,

834

LDL , 526

Le Berre , 387

Le Roch , 679

Leary , 514 ,

991

Lecithin , 861 , see also

Phosphatidyl choline

Lectin , 311 , 314 , 878

binding to liposomes , 878

gold labeled , 927

gold labeling of , 932

hydrazide activated , 968

plant toxins , 827

Lee , 27 , 29 , 189 , 243 , 254 , 302 ,

351 , 413 , 460 , 511 , 530 ,

550 , 552 , 658 , 754 , 939

LeFebvre , 308

Leffak

, 256

Lehrer , 521

Leitner ,

650

Lemieux , 666 , 671

Lennarz , 46

Leon , 355

Lerner , 287

Leserman , 879 , 882

Lesniak , 389

Leteux , 151 , 152 , 154 , 541 , 542

Lethias , 930

Leucine , 5

photoreactive diazirine

derivative of , 208

solvent exposed area , 30

Leucine zipper peptide

interaction with quantum

dots, 490

Leukemia virus , 256

Leung , 467

Levine , 25

Levison , 95

N

-Levulinoyl- D -mannosamine

(ManLev), 671

Lewis , 312

Li , 90 , 421 , 479 , 501 , 546 , 653 ,

667

7

Li , 825

Liang , 280

LiCl , 971

Liener , 128 , 162

Light chains , 784

Light microscopy , 924

Likhosherstov , 155

Lim , 485

Lin , 661 , 685

Lindersson , 308

Lindley , 120

Lindner , 650

Link , 681 , 683 , 684

Lipase , 687

Lipid A , 879

Lipid bilayers , 753

Lipid peroxidation , 655

Lipids , 859

activation and derivatization ,

869

azido analogs , 692

detergent-assisted

solubilization of , 862

functional derivatives , 869

functionalities , 869

gold labeled , 927

mechanical dispersion of , 862

periodate oxidation of

glycolipids, 870

protection from oxidation ,

861

Index 1173

solvent-mediated dispersion

of , 862

sphingosine derivatives , 866

types of , 864

Lipoic acid , 189

Lipopolysaccharides , 46 , 306

Lipoproteins , 20 , 254

gold labeled , 927

Liposome , 292 , 701

activation with SPDP , 876

adjuvant effect of , 879

antibody conjugates with , 824

attachment of haptens to

surface , 879

biotinylated , 883

chemical constituents of , 863

cholesterol incorporation , 868

conjugation

to

avidin , 883

proteins using glutaralde-

hyde , 891

proteins using iodoacetyl

groups , 898

proteins using SMCC , 897

proteins using SMPB , 895

using lectins , 878

with

antibodies , 881

antibodies using NHS-

palmitate , 886

antigens or haptens , 879

peptide haptens , 881

proteins , 870 , 886

proteins using avidin-

biotin interaction , 888

containing encapsulated

haptens , 879

cryoprotectants , 863

detergent-assisted

solubilization of , 862

encapsulation with hapten

molecules , 880

ﬁ lled with cytotoxic agents ,

824

freeze-fracture effects , 863

functional derivatives , 869

half-life in vivo

, 868

immunogen conjugates , 868

lipid components , 861

lyophilization of , 863 , 869 ,

871

mechanical dispersion

methods , 862

membrane ﬂ uidity and

permeability , 869

morphology , 858

periodate oxidation of , 870

preparation of , 861

preparation of conjugates and

derivatives , 858

protection from oxidation ,

861

reductive amination

conjugation , 893

size fractionation of , 863

solvent-mediated dispersion

of , 862

sphingolipid containing ,

866

use in immunoassays , 883

used as carriers , 747 , 753

Liposome immunosorbent assay

(LISA)

, 883

Liquid crystal state , 869

Lis , 366

Lissamine

hydrazide derivatives of , 427

Lissamine rhodamine B , 416 ,

429

comparison to Texas Red , 424

Lissamine rhodamine B sulfonyl

chloride , 423 , 916

general description and use ,

422 , 427

labeling of avidin with , 916

Liu , 176 , 251 , 287 , 293 , 319 ,

342 , 353 , 498 , 640 , 1028

Liver disfunction , 529

Liz-Marzan , 620

Ljungdahl , 1016

Lloyd , 904

Loh , 667

Loken , 461

Lomant , 171 , 172 , 235 , 238 ,

940

Lomant’s reagent , see DSP

Long , 192

Lonnerdal , 814

Lopatin , 575

Lord , 824

Lotan , 130 , 139

Louis , 256

Lövgren , 484

Low , 701

Low-density lipoprotein , see

LDL

Lowe , 59 , 62 , 171 , 223 , 224 ,

256 , 274 , 977 ,

989

Lu , 328 , 655 , 701

Lubin , 254 , 255

Lucifer Yellow , 455 , 457

comparison to Cascade Blue ,

453

Lucifer Yellow CH , 453

general description and use ,

459

Lucifer Yellow ﬂ uorophores

general description and use ,

457

Lucifer Yellow iodoacetamide

general description and use ,

458

Luduena , 275

Ludwig

, 219 , 251

Luminescence , 398 , 399 , 400 ,

914 , 998

Luminescence resonance energy

transfer (LRET) , 478

Luminol , 965

Lumper

, 256 , 258

Lundbald , 126

Luteinizing hormone  mRNA ,

531

lymphoblast receptors , 247

lymphocyte receptors , 395

lymphocytes , 246 , 310 , 511

B cells , 745 , 746

T cells , 745 , 746

T helper cells , 745

Lymphokine , 746

conjugates with antibodies ,

824

conjugation to toxins , 847

Lymphotoxin , 247

Lysine , 7 , 102 , 109 , 119 , 234 ,

529 , 534 , 801 , 900

blocking excess glutaraldehyde

with , 913

blocking of , 157

detection with TNBS , 128

1174 Index

Lysine ( continued )

for

constructing trifunctional

cross-linkers, 336

quenching glutaraldehyde

reactions, 798 , 800

guanidination of , 181

in

biocytin , 336 , 528

MAP , 755

oxidation of , 28

pKa , 9

reaction with

anhydrides , 178

glutaraldehyde , 134

haloacetyl compounds , 183

solvent exposed area , 30

Lysine nitrilotriacetic acid , see

NTA

Lysozyme , 230 , 335 , 945

M

2

C

2

H

general description and use ,

299

Machy , 879 , 882



2

-Macroglobulin

in cytotoxic conjugates , 825

SPDP , 837

Macrophages , 879

Maehashi , 645

Maggini , 629 , 631 , 632

Magic bullet , 824

Magnesium , 963

Magnesium ions , 970

Magnusson , 147

Mahal , 671

Mahan , 284

Major histocompatibility com-

plex, see MHC

Majoros , 365 , 371 , 372 , 373

Makarova , 620

Malachite green

similarity to rhodamine , 399

Malcolm , 982

Malcom

,57

Male , 746

Maleamic acid , 184 , 283 , 722 ,

767

Maleic acid , 106 , 159

Maleic acid imides , see

Maleimides

Maleic anhydride , 183

as reversible blocking agent ,

157

deblocking of acylated amines ,

107

for reversibly blocking amines ,

159

general description and use ,

107

2-methyl derivative , 108 , 159

Maleimide , 35 , 107 , 108 , 325 ,

406 , 411 , 689 , 749 , 788 ,

809 , 834 , 871 ,

895, 906

absorptivity of , 768

cyanine dye derivative , 470

derivative of Jeffamine , 948

dextran modiﬁ ed , 960

Diels-Alder reaction with , 210

hydrolysis of , 184 , 767

in

biotinylation reagents , 520

carbonyl and sulfhydryl

reactive cross-linkers ,

298

Diels-Alder reactions , 668

of

benzophenone-4-maleimide ,

330

biotin-BMCC , 520

BMH , 258

ﬂ uorescein-5-maleimide ,

408

GMBS , 292

M2C2H , 299

MBS , 286 , 852 ,

875

MPBH ,

298

NEM , 160

SMCC , 850

SMPB , 291 , 854

reactions of , 183

reactivity of , 283

relative stability , 276

stability of

in sulfo-SMCC , 766

in SMCC , 283

use in immunotoxin

conjugation, 847

Maleimide-mPEG

n

, 740

Maleimide-PEG

2

-biotin , 668

Maleimide-PEG

n

-biotin , 732

N -Maleimido-6-aminocaproyl

ester of 1-hydroxy-2-nitro-

4-benzene sulfonic acid , 948

m -Maleimidobenzoyl- N -hydrox-

ysuccinimide ester , see MBS

m -Maleimidobenzoyl- N -hydrox-

ysulfosuccinimide ester , see

Sulfo-MBS

N-(-Maleimidobutyryloxy)succi-

nimide ester , see GMBS

N -(  -Maleimidobutyryloxy)sulfo

succinimide ester , see Sulfo-

GMBS

4-( N -Maleimidomethyl)cyclohe-

xane-1-carboxyl-hydrazide ,

see M

2

C

2

H

4-(4- N -Maleimidophenyl)butyric

acid hydrazide , see MPBH

p -Maleimidophenyl isocyanate ,

see PMPI

N-  -Maleimidopropionic acid

(or 3-maleimidopropionic

acid), see BMPA

3-( N -Maleimidopropionyl)

biocytin

comparison to biotin-BMCC ,

521

3-Maleimidopropionyl chloride ,

630

Maleylation , 107

Malhotra , 984

Malignancies , 819

Malignant , 824

Mallett , 889

Mallia , 140

Malondialdehyde , 262

Malonic acid , 207

formed through diazopyruvate

photolysis, 322

Maltose

lactone derivative

, 367

Mamede , 379 , 383

Manabe , 824 , 857

Manak , 971 , 996

Manduchi , 380

Mandy , 256

Manevich , 1026

Manger , 155

Index 1175

Mann , 316

Mannich condensation , 801

with tyrosine , 11

Mannich reaction , 201 , 203 , 264

general description , 777

in hapten–carrier conjugation ,

776

Mannik , 801

Mannose , 21 , 38 , 46

azide derivative , 544

binding of ricin A chain , 830

dendrimer derivative , 366

reaction with phenylhydra-

zine , 42

Manshouri , 945

Manske , 830

MAP , 755

March , 347 , 612 , 667 , 680

Marchan , 668

Marcholonis , 555

Marcus , 223

Marfey , 260

Margel , 601

Marinetti , 330

Markwell , 255 , 551 , 555

2-( N -Morpholino)ethane

sulfonic acid , see MES

Martin , 287 , 291 , 292 , 485 ,

855 , 863 , 872 , 873 , 875 ,

889 , 895 , 896

Martinez-Ramon , 932

Maruyama , 194

Marwell , 553

Masamune , 101

Masri , 188

Mass normalization group , 660

Mass reporter group , 660

in PIR compounds , 1012

Mass spec , 709 , 1018

for analysis of PIR crosslinks ,

1012

using

heavy atom, deuterated

crosslinking agents ,

1008

ICAT reagents , 652

Mass spectrometry (MS) , 181 ,

649

Mass tag , 649 , 650

ECAT reagent , 657

isobaric , 659

types , 651

Massague , 310

Masuho , 830 , 846

Matheson , 48

Matrix-assisted laser desorption/

ionization (MALDI) , 649

Matteucci , 104

Matthay , 824 , 882

Matthews , 59

Mattiasson , 801

Mattoussi , 489

Mawas , 147

Mayhew , 862

Mazaitis , 549

MBS , 184 , 293 , 766

activation of

PE , 875

ricin , 854

analog of , 287

general description and use ,

286

in coupling antibodies to

liposomes , 883

liposome conjugation using ,

871

maleimide stability , 286

use in antibody–enzyme

conjugation , 788

MBS–DPPE , 875

McBroom

, 774

McCarron , 783 , 824

McCleary , 48

McDevitt , 284

McGinnis , 48

McGown , 400 , 419

McInnes , 531

McKinney , 403

McMahan , 1018

Means , 21

Meares , 170 , 498 , 501 , 657 ,

1032 , 1033 , 1036

Mecher , 638

Medintz , 489 , 490 , 492

Megathura crenulata , 748

Meige , 427

Meighen , 103

Meijer , 353 , 638

Meinhoffer , 175 , 260

Melcher , 964

Meldal , 683

Mengo virion , 239

Mentzer , 252 , 254 , 255

Mercaptans , 97

 -Mercaptoethanol , see

2-Mercaptoethanol

2-Mercaptoethanol , 89 , 97 , 161 ,

239

, 289 , 450 , 451 , 459 ,

523 , 555 , 765 , 768 , 971 ,

973

affect on aryl azides , 305

for quenching EDC reactions ,

222

general description and use ,

91

in

2-D electrophoresis , 93

gram-negative bacteria lysis

buffer , 92

OPA reagent , 93 , 128

quenching EDC reactions ,

763

SDS sample buffer , 93

reaction with maleimide-

activated BSA , 769

2-Mercaptoethansulfonic acid ,

706

2-Mercaptoethylamine , 84 , 88 ,

89 , 792 , 981 , 982

antibody reduction using , 436

as spacer in creating

iodoacetyl activated

liposomes , 899

for

cleavage of antibody

molecules , 786

reduction of F(ab  )

2

fragments , 786

general description and use ,

93

in

reduction of

antibodies , 789 , 790

F(ab  )

2

fragments , 809

Mercaptosuccinic acid , 426

11-Mercaptoundecanoic acid ,

385

Mercuration

of pyrimidines , 57

Meredith , 112

1176 Index

Merriﬁ eld , 224

MES , 218

messenger RNA , 51

Messer , 964

[Met5]- enkephalin

conjugation to

BSA , 757

KLH using EDC , 762

Metal chelate afﬁ nity

chromatography , 813 , 814

Metal chelators , 963

Metal ions , 498

Metal-Thiol Dative Bond , 188

Metals

immobilized , 814

Metasticies , 819

Methacrylate , 593

Methanethiolsulfonate group

(MTS), 122 , 342 , 371 , 1028

Methanol , 264 , 408 ,

861, 873

,

874 , 875 , 964

Methionine , 5 , 102 , 109 , 119

analogs , 684

oxidation of , 25

photoreactive diazirine

derivative of , 208

reaction with

cisplatin , 192

haloacetyl compounds , 182

solvent exposed area , 30

Metho p -toluene sulfonate , 55 ,

223

Methotrexate

dendrimer derivative , 371

N

4

-Methoxy-5,6-dihydrocyto-

sine-6-sulphonate, 976

8-Methoxypyrene-1,3,6-

trisulfonic acid , 453

Methoxysilanes , 563 , 567

Methoxytrityl oxazolidinone ,

631

Methyl 3-mercaptopropionimi-

date hydrochloride , 67

N -Methyl-3,4-fulleropyrrolidine ,

630

Methyl 4-benzoylbutyrate , 637

Methyl 4-mercaptobutyrimidate ,

see 2-Iminothiolane

Methyl Methanethiosulfonate

(MMTS), 163

N -Methyl piperazine , 662

Methyl sulfonate , 342 , 1028

N

7

-Methylguanine , 51

2-Methylmaleic anhydride , see

Citraconic anhydride

Methylene chloride , 555

Methylhydroxylamine , 976

Methylisourea , see o-Methyli-

sourea

o -Methylisourea , 181

o -Methylisourea hemisulfate ,

182

guanidination of lysine using ,

650

Metrizamide , 862

Metz , 223

Meurling , 498 , 825

Meyer , 690

Meyne , 1000

MgCl

2

, 971 , 973

MHC , 745

Mice , 750

Micelles , 859

inverted , 861

Michaela , 465

Michel , 383

Microinjection , 457

Microparticles , 582

surface area , 588

Microplate , 271

Microscopy , 254

electron , 924

ﬂ uroescence , 914

light , 924

use of avidin–biotin

interaction, 905

Microsomes , 254

Microspheres

polymeric , 588

Microtubules , 423 ,

407 ,

421

Mielke , 282

Mikhailov , 1016

Mikkelsen , 262

Miklis , 353

Mikolajczyk , 136

Millar , 247

Miller , 287

Min ami , 1026

Minard-Basquin , 380

Miron , 606 , 610 , 943 , 945

Mirzadeh , 383

Misawa , 24

Miskimins , 86

Mitchison , 421

Mitochondria , 423

Mitomycin-C , 954

Mitotic spindle assembly , 421

Mittal , 427

Mixed disulﬁ de

, 89 , 91 , 100 ,

185 , 187

Miyagi , 650

Miyakawa , 256

MLV , 754

Mochizuki , 319

Modeccin , 827 , 828

structure of , 827

Modiﬁ cation reagents

iodinatable , 556

Moebius , 316

Moeremans , 924

Moffatt oxidation

of mPEG , 950

Moi , 501

Mokotoff , 69

Molina-Bolıvar , 585

Mollusk , 748

Momordica Charantia seeds ,

827

Momordin , 827

Monaghan , 350

Mono-iodotyrosine , 548

Mono(lactosylamido) mono(su

ccinimidyl)suberate, 149 ,

150 , 369

dendrimer modiﬁ cation using ,

368

Monocarboxymethyllysine , 110 ,

183

Monoclonal antibodies , 783

activity loss of , 784

conjugation with toxins , 824

produced from hapten–carrier

conjugates, 747

recombinant , 826

toxin conjugates of , 827

Monoclonals

humanized mouse , 826

Monomeric avidin

immobilized , 539 , 653 , 693 ,

1026 , 1031

Index 1177

Monomethoxypolyethylene

glycol , see mPEG

Monooxygenase , 256

Monosaccharides

cyclic forms , 37

functional groups , 39

stereoisomers , 38

Monsan , 266 , 779

Montesano , 242

Moore , 256 , 258

Morales , 407 , 409

Moreland , 327

Morgan , 242 , 430

Moroder , 111

Moroi , 241 , 316

Moroney , 256 , 258 , 832

Morpurgo , 188

Morris , 928 ,

934

Morrison , 306 , 555 , 1000

Mosbach , 224 , 422 , 424

Mossberg , 418 , 424 , 429

Motta-Hennessy , 503

Moudrianokis , 254

Mourey , 353

Mouritsen , 210

Mouse IgG , 1807

Mouse IgG2a , 807

MPB-DPPE , 881

MPB–PE , 873

MPBH

general description and use ,

298

mPEG , 353 , 938 , see also PEG

activation with

CDI , 947

DSC ,

943

trichloro-s-triazine , 939

aldehyde derivative of , 950

anhydride modiﬁ cation of , 940

CDI activation of , 946

chloroformate derivative of ,

941

discrete modiﬁ cation agents ,

740

modiﬁ cation with succinic

anhydride , 946

Moffatt oxidation of , 950

succinimidyl carbonate deriva-

tive of , 941

succinylation of , 941

mPEG-maleimide , 668

mPEG-thiol , 709

mRNA , 51 , 438 , 531

MTS-ATF-Biotin , 1028

general description and use ,

341

MTS-ATF-LC-Biotin , 1028

general description and use ,

341

Muckerheide , 116 , 750 , 879

Mucoproteins , 20 , 46

Mudd , 255

Muir , 685 , 701 , 703 , 706

Mukkala , 502

Muller , 1009

Muller-Eberhard , 857

Mullis , 970

Multidimensional protein

identiﬁ cation technology

(MudPIT) , 661

Multilamellar vesicles , 860 ,

Hermanson G. Bioconjugate Techniques, Second Edition

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