[15:26 13/3/03 n:/3991 RUSSELL.751/3991-002.3d] Ref: 3991 Whisky Chapter 2 Page: 50 26-73
in the endosperm and have no direct GA requirement. During germi nation,
latent b-amylase is converted into the active form by the action of proteolytic
enzymes.
Fermentable extract is almost exclusively derived from the starchy endo-
sperm of the germinated grain. Release of this material, by the process of
modification, depends on the combined action of several hydrolytic enzymes
on cell walls and matrix proteins. In the endosperm, starch occurs as large (20–
30 mm) and small (1–5 mm) granules. Aspects of their release are well covered
in the literature (Bourne, 1999; Lynn et al., 1999; Stuart et al., 1999). Although
the small granules are more numerous, they represent a minor weight propor-
tion of the total starch. Both types of granule are embedded in a matrix of
reserve protein composed of hordeins and glutelins. This conglomerate of
starch and protein is contained within a cellular envelope composed of 70–
75 per cent glucan, 20–25 per cent pentosan, and other minor components. Of
the cell wall glucan, 70 per cent is b1,4 linked, the remai nder being linked in
the b1,3 position. Modification of the endosperm follows the release of b-
glucanases, end o-proteases and pentosanases from the aleurone layer. A sig-
nificant amount of b-glucan is degraded during malting, along with 35–40 per
cent of the reserve protein, but less than 15 per cent of the starch is broken
down. Incomplete modification of the endosperm will result in reduced
extraction of starch during mashing, and a reduced susceptibility of the starch
to enzymic attack.
In terms of modification, much research effort has been made to isolate the
factors that distinguish good malting barley. Malting quality is a varietal char-
acteristic, and is not related simply to either glucan or protein content. Malting
varieties usually modify faster than non-malting varieties, even when their TN
content is higher. Traditionally, mealiness and steeliness in the barley endo-
sperm have been used to judge quality. Steely grains (which malt less readily)
generally contain more nitrogen than corresponding mealy grains, but the
relationship between endosperm nitrogen and steeliness is not consistent
between varieties. It is possible that in poor malting varie ties there are limited
areas of high-nitrogen endosperm that display an extreme degree of steeliness
and modify at a slower rate. The polypeptide composition of reserve proteins
(hordeins) in different varieties appears to be related to their malting potential
(Wainwright, 1979). High glucan content does not always signify poor malting
quality; barleys with relatively high levels of cell wall materials modify suc-
cessfully if adequate b-glucanase levels can be achieved. In assessing this
provision, it is important to distinguish between free enzyme and unsecreted
non-functional enzyme (Palmer et al., 1985). The application of electron micro-
scopy has had a limited success in relating endosperm microstructure to malt-
ing quality. Within the endosperm, the packing density of the starch-
containing ce lls varies, thus affecting ease of hydration, and differences are
apparent between varieties in the density of the small starch granule-protein
matrix. In areas of well-modified endosperm, cell walls are absent and, the
amount of protein matrix is greatly reduced.
Increasing the temperature of germination initially improves the rate of
modification, but final extracts are lower. However, the modification value
50 Whisky: Technology, Production and Marketing