During anaerobic digestion, energy is released, and much of the volatile
organic matter is converted to methane, carbon dioxide, and water. Thus
little carbon and energy are available to sustain further biological activ-
ity, and the remaining residuals are rendered stable.
Anaerobic digestion involves three basic successive phases of fer-
mentation: hydrolysis, acid formation, and methane formation (WEF
and ASCE, 1996b; US EPA, 1991). In the first phase of digestion, extra-
cellular enzymes (enzymes operating outside the cells) break down com-
plex organic substances (proteins, cellulose, lignins, lipids) into soluble
organic fatty acids, alcohols, carbon dioxide, and ammonia.
In the second phase, acid-forming bacteria, including facultative bac-
teria, convert the products of the first stage into short-chain organic
acids such as acetic acid, propionic acid, other low molecular weight
organic acids, carbon dioxide, and hydrogen. These volatile organic acids
tend to reduce the pH, although alkalinity buffering materials are also
produced. Organic matter is converted into a form suitable for break-
down by the second group of bacteria.
The third phase, strictly anaerobic, involves two groups of methane-
forming bacteria (methanogens). One group converts carbon and hydro-
gen to methane. The other group converts acetate to methane, carbon
dioxide, and other trace gases. Both groups of bacteria are anaerobic.
Closed digesters are used for anaerobic digestion. It should be noted that
many authors consider only two phases (excluding phase 1 above).
The most important factors affecting the performance of anaerobic
digesters are solids residence time, hydraulic residence time, tempera-
ture, pH, and toxic materials. Methanogens are very sensitive to envi-
ronmental conditions. Anaerobic digesters are usually heated to
maintain a temperature of 34 to 36⬚C (94 to 97⬚F). The methane bacte-
ria are active in the mesophilic range (27 to 43⬚C, 80 to 110⬚F). They have
a slower growth rate than the acid formers and are very specific in food
supply requirements. The anaerobic digester (two-stage) usually pro-
vides 10 to 20 days’ detention of sludge (WEF and ASCE, 1996b).
Optimum methane production typically occurs when the pH is main-
tained between 6.8 and 6.2. If the temperature falls below the operat-
ing range and/or the digestion times falls below 15 days, the digester
may become upset and require close monitoring and attention.
If concentrations of certain materials such as ammonia, sulfide, light
metal cations, and heavy metals increase significantly in anaerobic
digesters, they can inhibit or upset the process of performance.
The key parameter for digester sizing is solids residence time. For
digester systems without recycle, there is no difference between SRT and
HRT. Volatile solids loading rate is also used frequently as a basis for
design. Typically, design SRT values are from 30 to 60 days for low-rate
digesters and 10 to 20 days for high-rate digesters (WEF and ASCE, 1991b).
Wastewater Engineering 813