BD Diagnostic Systems (publ.). Difco Manual (Manual of Microbiological Culture)

Подождите немного. Документ загружается.

The Difco Manual 811

Section VI

Color Cover Page

The Difco Manual 813

Agar Selection Guide

APPLICATIONS

Agar, Granulated

Agar, Technical

Bacto

Agar

BACTERIOLOGICAL USES OF AGARS

APPLICATIONS

Agar, Granulated

Agar, Technical

Bacto

Agar

NON-BACTERIOLOGICAL USES OF AGARS

Immunodiffusion ++

Electrophoresis ++

Tissue Culture, mammalian ++

Tissue Culture, plant ++ +

Histology, tissue embedding + ++

Histology, bone marrow embedding ++ +

Insect growth substrate ++

Auxotrophic studies ++ +

Bacteriology, research ++ +

Bacteriology, general purpose ++ +

Bacteriophage studies ++

Biotechnology production + ++ +/–

General microbial production + ++ +/–

Growth of fastidious organisms ++

Identification of pathogenic organisms ++ + +/–

Microaerophilic studies ++

Molecular genetics ++ +

Prepared plate manufacture + ++ +

Quality control, production ++ + +/–

Quality control, environmental + ++ +/–

Transformation of bacteria ++

Transformation of yeast ++

Key

++ Recommended

+ Suitable

+/– Marginal

Section VI Agar Selection Guide

814 The Difco Manual

Anaerobe Broth MIC

Anaerobic Agar

Blood Culture Bottles Columbia Broth w/CO

Blood Culture Bottles Columbia Broth w/SPS and CO

Blood Culture Bottles Fluid Thioglycollate Medium w/SPS and CO

Blood Culture Bottles Thioglycollate w/CO

Blood Culture Bottles Thioglycollate w/SPS and CO

Brain Heart Infusion Agar

Brewer Anaerobic Agar

Brewer Thioglycollate Medium

CHO Medium Base

Clostridium Difficile Antimicrobic Supplement CC

Cooked Meat Medium

ESP Anaerobic Broth

Differential Reinforced Clostridial Agar

Fluid Thioglycollate Medium

Liver Veal Agar

McClung Toabe Agar Base

NIH Thioglycollate Broth

Reinforced Clostridial Medium

Schaedler Agar

Schaedler Broth

SFP Agar Base

Sterility Bottles with Septum Fluid Thioglycollate Medium

Sterility Bottles with Screw Cap Fluid Thioglycollate Medium

SPS Agar

Sulfite Agar

Thioglycollate Medium USP Alternative

Thioglycollate Medium w/o Dextrose

Thioglycollate Medium w/o Dextrose or Indicator

Thioglycollate Medium w/o Indicator

Wilkens-Chalgren Agar

ANAEROBES - GENERAL

Anaerobes - General

Anaerobes - General Section VI

The Difco Manual 815

Antimicrobic Selective Agents For Culture Media

Cefsulodin-novobiocin 4 mg/2.5 mg Yersinia Antimicrobic Supplement CN

Ceftozidime 40 mg Palcam Antimicrobic Supplement

Chloramphenicol 0.05 g Rose Bengal Antimicrobic Supplement C

Chlortetracycline (Aureomycin

) 25 mg Antimicrobic Vial A

Colistin Sulfate - Nystatin - Vancomycin 7,500 mcg/12,500 units/ Antimicrobic Vial CNV

3,000 mcg

Colistin Sulfate - Nystatin - Vancomycin 7,500 mcg/12,500 units/ Antimicrobic Vial CNVT

Trimethoprim 3,000 mcg/5,000 mcg

(10 ml vial)

Cyclohexamide - Colistin Sulfate - 400 mg/20 mg/5 mg/2 mg/ Oxford Antimicrobic Supplement

Acriflavine - Cefotetan - Fosfomycin 10 mg

Cycloserine - Cefoxitin 125 mg/ 5 mg Clostridium Difficile Antimicrobic

Supplement CC

Kanamycin 25,000 mcg Antimicrobic Vial K

Moxalactam 20 mg Moxalactam Antimicrobic Supplement

Moxalactam - Colistin Sulfate 20 mg/10 mg Modified Oxford Antimicrobic

Supplement

Novobiocin 20 mg Novobiocin Antimicrobic Supplement

Oxytetracycline 100 mg Antimicrobic Vial Oxytetracycline

Polymyxin B 30,000 units Antimicrobic Vial P

Potassium Tellurite Solution 1% 1% Chapman Tellurite Solution

Potassium Tellurite Solution 3.5% 3.5% Potassium Tellurite Solution 3.5%

Sodium 7- ethyl - 2 - methyl - XLT4 Supplement

4 - undecyl sulfate

AGENT CONCENTRATION PER VIAL PRODUCT NAME

Section VI Antimicrobic Selective Agents For Culture Media

816 The Difco Manual

Cosmetic Testing

Gram-Negative Screening

Pseudomonas

Isolation

Staphylococcus

Sterility Testing

Yeast & Mold Isolation

AC Broth ✔

AC Broth Medium w/o Dextrose

†

✔

Cetrimide Agar Base/PSEUDOSEL Agar

†

✔

RODAC

™

Plates ✔

D/E Neutralizing Agar

†

✔

D/E Neutralizing Broth

†

✔

EMB Agar/Eosin Methylene Blue Agar Modified

†

✔

Fluid Thioglycollate Medium

†

✔

HC Agar Base

†

✔

HYcheck ✔

Letheen Agar

†

✔

Letheen Broth/Letheen Broth AOAC

†

✔

m Staphylococcus Broth ✔

MacConkey Agar

†

✔

Malt Agar

†

✔

Malt Extract Agar ✔

Malt Extract Broth

†

✔

Mannitol Salt Agar

†

✔

Microbial Content Test Agar/

TRYPTICASE

™

Soy w/Lec. poly.

†

✔

Modified Letheen Agar ✔

Modified Letheen Broth ✔

Mycological Agar/MYCOPHIL

™

Agar

†

✔

Mycobiotic Agar/MYCOPHIL Agar

†

✔

Neutralizing Buffer

†

✔

Phenylethanol Agar/Phenylethyl Alcohol Agar

†

✔

Potato Dextrose Agar

†

✔

Pseudomonas Agar F/Flo Agar

†

✔

Pseudomonas Agar P/Tech Agar

†

✔

Pseudomonas Isolation Agar/Pseudomonas ISO

†

✔

Sabouraud Dextrose Agar

†

✔

Staph Latex Test Kit/STAPHYLOSLIDE

™

Test Kit

†

✔

Staphylococcus Medium 110/Staphylococcus Agar #10

†

✔

Sterility Test Bottles, Prepared

†

✔

TAT Broth Base

†

✔

Tryptic Soy Broth/ TRYPTICASE

™

Soy Broth

†

✔

VJ Agar/Vogel & Johnson Agar

†

✔

PRODUCTS

Environmental

APPLICATIONS

Cosmetic Testing Section VI

(See also Environmental Sampling

and Disinfectant Testing)

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 817

Dairy Product Testing - Products and Applications

Section VI Dairy Product Testing - Products and Applications

Antibiotic Medium 1

†

✔

Antibiotic Medium 4

†

✔

Azide Blood Agar Base

†

✔

Brilliant Green Bile 2%/Brilliant Green Bile Broth

†

✔

Bryant and Burkey Medium ✔

Concentration Disks 1/2" Penase ✔

Concentration Disks 1/2" ✔

Penicillin 0.05 Unit/PM Discs, 0.05, 1/4" Taxo

™†

Concentration Disks 1/2" Sterile Blanks,

Antibiotic Detect Disc 1/2" Taxo

†

✔

Desoxycholate Lactose Agar

†

✔

D/E Neutralizing Agar

†

✔

D/E Neutralizing Broth

†

✔

EC Medium w/MUG/EC Broth w/ MUG

†

✔

m FC Agar/M-FC Agar

†

✔

Fraser Broth Base and/Fraser Broth Base Modified

†

✔

Fraser Broth Supplement/Fraser Broth Additive

†

✔

HYcheck

™

✔

LPM Agar Base

†

✔

Lactose Broth

†

✔

Lauryl Tryptose Broth w/MUG/

Lauryl Sulfate Broth w/ MUG

†

✔

Letheen Agar

†

✔

Letheen Broth/Letheen Broth AOAC

†

✔

Levine EMB Agar/Levine Eosin Methylene Blue Agar

†

✔

Listeria Antisera ✔

M17 Broth

†

✔

Malt Extract Agar

†

✔

Malt Extract Broth

†

✔

McBride Listeria Agar

†

✔

Microbial Content Test Agar/

Trypticase

™

Soy Agar w/Lec & Polysorbate

†

✔

Milk Agar IDF Formulation ✔

Modified Listeria Enrichment Broth ✔

PRODUCTS

Beta-Lactams in Milk

Environmental

Standard Plate Count

Yeast & Mold Analysis

Streptococcus

Detection

APPLICATIONS

Clostridium

Coliform Analysis

Listeria

Analysis

Lipolytic Microorganisms

†

Available from Difco & Becton Dickinson Microbiology Systems.

818 The Difco Manual

Dairy Product Testing - Products and Applications

Dairy Product Testing - Products and Applications Section VI

PRODUCTS

Beta-Lactams in Milk

Environmental

Standard Plate Count

Yeast & Mold Analysis

Streptococcus

Detection

APPLICATIONS

Clostridium

Coliform Analysis

Listeria

Analysis

Lipolytic Microorganisms

Modified Oxford Antimicrobic

Supplement ✔

Motility Test Medium

†

✔

Moxalactam Antimicrobic Supplement ✔

Neutralizing Buffer

†

✔

Nutrient Agar

†

✔

Nutrient Broth

†

✔

Oxford Antimicrobic Supplement ✔

Oxford Medium Base/ Oxford Agar Base Modified

†

✔

PALCAM Medium Base and

PALCAM Antimicrobic Supplement ✔

PM Indicator Agar ✔

PM Negative Control ✔

PM Positive Control ✔

Penase Concentrate/Penicillinase Concentrate

†

✔

Phenylethanol Agar/Phenylethanol Alchol Agar

†

✔

Plate Count Agar/Standard Methods Agar

†

✔

Potato Dextrose Agar

†

✔

Spirit Blue Agar w/Lipase Reagent ✔

Strep Grouping Kit

†

✔

Subtilis Spore Suspension ✔

Thermospore Suspension PM ✔

Tryptic Soy Broth/TRYPTICASE

™

Soy Broth

†

✔

Tryptone Glucose Extract Agar/

TRYPTICASE Glucose Extract Agar ✔

UVM Modified Enrichment Broth/

UVM Mod. Listeria Enrichment Broth

†

✔

Universal Preenrichment Broth ✔

Violet Red Bile Agar

†

✔

Violet Red Bile Agar w/MUG

†

✔

Yeast Extract Glucose Chloramphenicol Agar ✔

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 819

*Custom formulations and packaging are also available.

Environmental Sampling and Disinfectant Testing

Fermentation Products

Section VI Environmental Sampling and Disinfectant Testing & Fermentation Products

CULTURE MEDIA HYGIENE CONTACT SLIDES

HYcheck™ D/E Neutralizing Agar

HYcheck for Disinfection Control

HYcheck for Enterobacteriaceae

HYcheck for Plate Count Agar with TTC

HYcheck for Total Count

HYcheck for Yeasts and Molds

HYcheck for Yeasts and Molds with TTC

Aseptic Commissioning Medium

Bushnell-Haas Broth

D/E Neutralizing Agar

†

D/E Neutralizing Broth

†

Disinfectant Test Broth AOAC

Egg Meat Medium

Letheen Agar

†

Letheen Broth/Letheen Broth AOAC

†

Microbial Content Test Agar /Trypticase

™

Soy Agar

w/ Lec. & Polysorbate

†

Neutralizing Buffer

†

Synthetic Broth AOAC

CONTACT PLATES SETTLING PLATES (TRIPLE WRAPPED)

DOUBLE WRAPPED (IRRADIATED)

D/E Neutralizing Agar

Sabouraud Dextrose Agar w/Lecithin and

Polysorbate 80

Standard Methods Agar

Tryptic Soy Agar w/Lecithin and Polysorbate 80

STERILE 100mm OR 150mm

Sabouraud Dextrose Agar

Tryptic Soy Agar

Tryptic Soy Agar w/Lecithin and Polysorbate 80

Todd Hewitt Broth

†

Tryptic Soy Broth/TRYPTICASE

™

Soy Broth

Tryptic Soy Broth w/o Dextrose/

TRYPTICASE Soy Broth w/o Dextrose

†

Tryptone/Select TRYPTICASE

Peptone

†

Tryptose/ POLYPEPTONE

™

Peptone

†

Tryptose Phosphate Broth

†

Yeast Extract/Select Yeast

Extract

†

Yeast Extract, Technical

†

Beef Extract, Desiccated

†

Brain Heart Infusion

†

Brucella Broth

†

Casamino Acids/Select

Casamino Acids

†

Casamino Acids, Technical

†

Casitone

Eugon Broth/EUGONBROTH

™

Gelatone/Gelysate Peptone

†

Heart Infusion Broth

†

M Broth

†

FERMENTATION*

Malt Extract

†

Neopeptone

Peptamin

Peptone, Bacto

Peptone Bacteriological, Technical

Proteose Peptone/Meat Peptone

†

Proteose Peptone No. 2

Proteose Peptone No. 3

Soytone/PHYTONE

™

Peptone

†

TC Lactalbumin Hydrolysate

TC Yeastolate/Yeastolate, TC

†

Available from Difco & Becton Dickinson Microbiology Systems.

820 The Difco Manual

Escherichia coli

- Products and Applications

Escherichia coli-Products and Applications Section VI

PRODUCTS

Nonselective Media

APPLICATIONS

Brain Heart Infusion Agar

†

✔

BAGG Broth ✔

Brilliant Green Bile Agar

†

✔

CULTURESWAB

™

Transport System Amies Medium ✔

CULTURESWAB Transport System Amies Medium ✔

w/o Charcoal/CULTURETTE

™

Amies w/o Charcoal

†

CULTURESWAB Transport System Cary-Blair Medium/

Anaerobic CULTURETTE Cary-Blair Single

†

✔

CULTURESWAB Transport System Stuart’s Medium

Modified/CULTURETTE Modified Stuart's Medium

†

✔

EC Medium w/MUG

†

✔

EMB Agar/Eosin Methylene Blue Agar Modified

†

✔

E. coli H Antiserum H7

†

✔

E. coli O Antiserum O157

†

✔

m Endo Agar LES/M-Enda Agar LES

†

✔

m Endo Broth MF

/M-Endo Broth ✔

EZ Coli™ Rapid Detection System for E. coli 0157 ✔

Lauryl Tryptose Broth w/MUG/Lauryl Sulfate Broth w/MUG

†

✔

MacConkey Agar

†

✔

MacConkey Sorbitol Agar/MacConkey II Agar w/Sorbitol

†

✔

Nutrient Agar

†

✔

Nutrient Agar w/MUG

†

✔

Transport Medium Amies ✔

Transport Medium Stuart

†

✔

Transport Medium Amies w/o Charcoal ✔

Tryptic Soy Agar/TRYPTICASE

™

Soy Agar

†

✔✔

TSA Blood Agar Base ✔✔

Veal Infusion Agar

†

✔✔

Violet Red Bile Agar

†

✔

Violet Red Bile Agar w/MUG

†

✔

Purity Plate

Rapid Test

Selective-Differential

Media

Serology

Specimen Collection

and Shipment

Purple Agar/Broth Base

Adonitol –

Dulcitol V

Glucose +

Inositol –

Lactose V

Mannitol +

Salicin V

Sucrose V

Ammonium Citrate –

Gelatin –

S–

Indole +

KCN –

Methyl Red +

Phenylalanine –

Sodium Malonate –

Tryptone Water +

Urease –

Voges-Proskauer –

Key

– Negative

+ Positive

d Delayed

V Variable +, d or –

BIOCHEMICAL TESTS

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 821

Food and Beverage Testing - Products and Applications

Section VI Food and Beverage Testing - Products and Applications

†

Available from Difco & Becton Dickinson Microbiology Systems.

A-1 Medium/A-1 Broth

†

✔

APT Agar

†

✔

APT Broth

†

✔

Baird-Parker Agar Base w/EY Tellurite Enrichment

†

✔

Bismuth Sulfite Agar

†

✔

Brilliant Green Agar

†

✔

Brilliant Green Agar Modified (Edel-Kampelmacher) ✔

Brilliant Green Bile 2%/Brilliant Green Bile Broth 2%

†

✔

Brucella Agar

†

✔

Brucella Broth

†

✔

Bryant and Burkey Medium ✔

Buffered Peptone Water

†

✔

Campylobacter Agar Kit Blaser/Campylobacter Agar

w/5 Antimicrobics and 10% Sheep Blood

†

✔

Campylobacter Agar Kit Skirrow ✔

Campylobacter Agar Base ✔

Coagulase Plasma (Rabbit)

†

✔

Coagulase Plasma EDTA (Rabbit)/

Coagulase Plasma, Rabbit w/ EDTA

†

✔

Cooke Rose Bengal Agar ✔

Cooked Meat Medium

†

✔

D/E Neutralizing Agar

†

✔

D/E Neutralizing Broth

†

✔

DNAse Test Agar

†

✔

DNAse Test Agar w/Methyl Green/

DNAse Test Agar w/Toluidine Blue

†

✔

DRBC Agar ✔

Demi-Fraser Broth Base ✔

Desoxycholate Citrate Agar Hynes

†

✔

Differential Reinforced Clostridial Agar ✔

EC Medium/EC Broth

†

✔

EC Medium with MUG/EC Broth w/MUG

†

✔

Elliker Broth

†

✔

m Endo Agar LES

†

✔

m Endo Broth MF

/ mEndo Broth, ALPHA

†

✔

EZ Coli

™

Rapid Detection System ✔

Fluid Thioglycollate Medium

†

✔

Fraser Broth Base/Fraser Broth Base Modified

†

✔

Fraser Broth Supplement/Fraser Broth Base Supplement

†

✔

Hektoen Enteric Agar

†

✔

HYcheck

™

D/E Neutralizing Agar ✔

HYcheck for Disinfection Control ✔

HYcheck for Enterobacteriaceae ✔

HYcheck for Total Count ✔

HYcheck for Yeasts and Molds ✔

HYcheck for Yeasts and Molds w/TTC ✔

LPM Agar Base

†

✔

PRODUCTS

APPLICATIONS

Bacillus

Beverage Analysis

Campylobacter

Analysis

Clostridium

Coliform Analysis

Environmental

Lactobacillus

Yeast & Mold Analysis

Yersinia

spp.

Vibrio

Salmonella/Shigella

spp.

Staphylococcus

Total Plate Count

Listeria

Analysis