BD Diagnostic Systems (publ.). Difco Manual (Manual of Microbiological Culture)

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832 The Difco Manual

Pharmaceutical Testing - Products and Applications

Micro Assay Culture Agar ✔

Micro Inoculum Broth ✔

Neurospora Culture Agar ✔

Niacin Assay Medium ✔

NIH Thioglycollate Medium

†

✔

Panthenol Assay Medium ✔

Pantothenate Assay Medium ✔

Pantothenate Medium AOAC USP ✔

Pyridoxine Y Medium ✔

Riboflavin Assay Medium ✔

Sterility Bottles w/ Screw Cap Fluid

Thioglycollate Medium/

Fluid Thioglycollate Medium

†

✔

Sterility Bottles w/ Screw Cap Tryptic

Soy Broth/Trypticase

™

Soy Broth

†

✔

Sterility Bottles w/ Septum Fluid A/

Peptone Water (0.1%)

†

✔

Sterility Bottles w/ Septum Fluid D/

Peptone Water (0.1%) w/Polysorbate

†

✔

Sterility Bottles w/ Septum Fluid

Thioglycollate Medium

†

✔

Sterility Bottles w/ Septum Tryptic Soy Broth/

Septum Fluid Thioglycollate Medium

†

✔

Thiamine Assay Medium LV ✔

Tryptic Soy Broth/Trypticase

™

Soy Broth

†

✔

Vitamin B12 Assay Medium ✔

Pharmaceutical Testing - Products and Applications Section VI

Vitamin/Amino Acid Assay

APPLICATIONS

PRODUCTS

Sterility Testing

Antibiotic Assay

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 833

Salmonella

- Products and Applications

APPLICATIONS

Primary Differential Media

Streak lactose negative

colonies on

PRODUCTS

Enrichment

Adonitol/Adonitol CP

†

–

Alginate –

Arginine + or (+)

Bismuth Sulfite Agar

†

✔

BG Sulfa Agar ✔

Brilliant Green Agar

†

✔

Brilliant Green Agar Modified (Edel-Kampelmacher) ✔

Desoxycholate Agar

†

✔

Desoxycholate Citrate Agar

†

✔

Dulcitol (+) d

EMB Agar

†

✔✔

Erythritol –

Esculin –

Gelatin –

Glucose +

GN Broth Hajna

†

✔

Hektoen Enteric Agar

†

✔

S +(–)

Individual O and H Antisera ✔

Indole –

Inositol d

KCN –

Lactose –

Lysine +

Lysine Iron Agar

†

✔

MacConkey Agar

†

✔✔

MacConkey Agar CS ✔

Methyl Red +

Highly Selective Media

Key

– Negative

+ Positive

d Delayed

(+) Variable

(–) Variable

Serological Identification

Differential Agar

Biochemical Tests

Section VI Salmonella- Products and Applications

†

Available from Difco & Becton Dickinson Microbiology Systems.

834 The Difco Manual

MIO Medium ✔

Muller Kauffmann Tetrathionate Broth Base ✔

Nitrate Reduction +

Ornithine + or (+)

Oxidase –

Phenylalanine –

Polyvalent Antisera ✔

Raffinose –

Salicin –

SBG Enrichment ✔

SBG Sulfa Enrichment ✔

Selenite Broth ✔

Selenite Cystine Broth

†

✔

SIM Medium

†

✔

Simmons Citrate

†

(+) d

Sodium Malonate –

SS Agar

†

✔

Sucrose –

m Tetrathionate Broth Base ✔

Tetrathionate Broth Base

†

✔

Triple Sugar Iron Agar

†

✔

Universal Preenrichment Broth ✔

Urea Agar Base

†

✔

Urease –

VP –

XLD Agar

†

✔

XLT4 Agar Base and XLT4 Supplement ✔

Salmonella

- Products and Applications

Salmonella - Products and Applications Section VI

APPLICATIONS

Primary Differential Media

Streak lactose negative

colonies on

PRODUCTS

Enrichment

Highly Selective Media

Key

– Negative

+ Positive

d Delayed

(+) Variable

(–) Variable

Serological Identification

Differential Agar

Biochemical Tests

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 835

APPLICATIONS

PRODUCTS

Acetate Differential Agar ✔

Adonitol – –

Arabinose d +

Arginine Dihydrolase d d

Christensen Citrate – d

Desoxycholate Agar ✔

Desoxycholate Citrate Agar ✔

Dulcitol d d

EMB Agar

†

✔✔

Gas from Glucose –(1) –

Gelatin (22°C) – –

GN Broth Hajna

†

✔

Hektoen Enteric Agar

†

✔

S––

Indole – or+ +

Inositol – –

KCN – –

Lactose –(1) d

Lysine Decarboxylase – d

Lysine Iron Agar

†

✔

MacConkey Agar

†

✔✔

MacConkey Agar CS ✔

Malonate – –

Maltose d +

Mannitol + or– +

Methyl Red + +

Motility – –

Shigella

and

Alkalescens-Dispar

Group -

Products and Applications

Key

– Negative

+ Positive

d Different reactions

(1) Certain biotypes of S. flexneri produce gas; cultures of S. sonnei ferment lactose and sucrose slowly and decarboxylate ornithine.

(–) Variable

Biochemical

Tests

Shigella

A-D Group

Enrichment

Primary Differential

Media

Primary Plating Media

Streak lactose

negative colonies on

Section VI Shigella and Alkalescens-Dispar Group - Products and Applications

†

Available from Difco & Becton Dickinson Microbiology Systems.

836 The Difco Manual

Shigella

and

Alkalescens-Dispar

Group -

Products and Applications

Shigella and Alkalescens-Dispar Group - Products and Applications Section VI

APPLICATIONS

PRODUCTS

Biochemical

Tests

Shigella

A-D Group

Enrichment

Primary Differential

Media

Primary Plating Media

Streak lactose

negative colonies on

Mucate – d

Ornithine Decarboxylase d d

Phenylalanine – –

Raffinose d d

Rhamnose d d

Salicin – –

Simmons Citrate Agar

†

––

Sodium Acetate – +(+)

Sucrose –(1) d

Triple Sugar Iron Agar

†

✔

Urease – –

Voges-Proskauer – –

XLD Agar

†

✔

Xylose d +

Key

– Negative

+ Positive

d Different reactions

(1) Certain biotypes of S. flexneri produce gas; cultures of S. sonnei ferment lactose and sucrose slowly and decarboxylate ornithine.

(–) Variable

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 837

AC Broth

AC Medium

Agar Medium No. F

Brewer Thioglycollate Medium

Fluid Thioglycollate Medium

†

Fluid Thioglycollate Medium w/Beef Extract

†

Fluid Thioglycollate Medium w/K Agar

NIH Thioglycollate Medium

Sterility Bottles w/Screw Cap Fluid

Thioglycollate Medium

Sterility Bottles w/Screw Cap Tryptic Soy Broth

Sterility Bottles w/Septum Fluid A

Sterility Bottles w/Septum Fluid D

Sterility Bottles w/Septum Fluid Thioglycollate

Medium

Sterility Bottles w/Septum Tryptic Soy Broth

Thioglycollate Medium w/o Dextrose

Thioglycollate Medium w/o Indicator

Thioglycollate Medium w/o Dextrose or Indicator

Tryptic Soy Broth

See also: Environmental Sampling Section of the Reference Guide

STERILITY TESTING, MANUAL

See ESP, Industrial Applications

Sterility Testing

Section VI Sterility Testing

STERILITY TESTING, AUTOMATED

†

Available from Difco & Becton Dickinson Microbiology Systems.

838 The Difco Manual

Brain Heart Infusion

†

✔

Brain Heart Infusion Agar

†

✔

Brain Heart Infusion Agar and Clostridium

Difficile Antimicrobic Supplement CC

†

✔

Brucella Agar

†

✔

Brucella Broth

†

✔

Brucella Antisera ✔

Cellmatics™ Viral Transport Pack/

CULTURETTE

™

Viral Single Swab

†

✔

Cetrimide Agar Base/PSEUDOSEL

™

Agar

†

✔

Cooked Meat Medium

†

✔

CULTURESWAB™ Amies Medium ✔

CULTURESWAB Amies Mediumw/o Charcoal/

CULTURETTE Amies w/o Charcoal

†

✔

CULTURESWAB Cary-Blair Medium/Anaeno

CULTURETTE Cary-Blair Single

†

✔

CULTURESWAB Perinasal Swab w/Amies Medium ✔

CULTURESWAB Urethral Swab w/Amies Medium ✔

CULTURESWAB Stuart’s Medium Modified/

CULTURETTE Modified Stuart's Medium

†

✔

Cystine Heart Agar ✔

EMB Agar/Eosin Methylene Blue Agar Modified

†

✔

Eugon Agar/EUGONAGAR

™†

✔

Eugon Broth/EUGONBROTH

™†

✔

Fletcher Medium Base ✔

Fluid Thioglycollate Medium

†

✔

Francisella Tularensis Antigen ✔

Francisella Tularensis Antiserum

†

✔

Leptospira Enrichment EMJH ✔

Leptospira Medium Base EMJH ✔

Levine EMB Agar/Levine Eosin Methylene Blue Agar

†

✔

MacConkey Agar ✔

McClung Toabe Agar Base

†

✔

Pseudomonas Agar F/Flo Agar

†

✔

Pseudomonas Agar P/Tech Agar

†

✔

Pseudomonas Isolation Agar/Pseudomonas ISO

†

✔

Reinforced Clostridium Medium/

Reinforced Clostridum Agar

†

✔

Veterinary Testing - Products and Applications

PRODUCTS

APPLICATIONS

Collection/Transport

Bacterial

Collection/Transport, Viral

Clostridium

Francisella

Leptospira

Pseudomonas

General Purpose Bacterial

Culture Media

Brucella

Gram Negative Enteric

Bacteria (General)

Veterinary Testing - Products and Applications Section VI

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 839

Azide Blood Agar Base

†

✔

Baird-Parker Agar Base

w/ EY Tellurite Enrichment

†

✔

Bismuth Sulfite Agar

†

✔

Blood Culture Bottles Brain Heart

Infusion w/ PAB, SPS + CO

✔

Blood Culture Bottles Columbia

Broth w/CO

✔

Blood Culture Bottles Columbia

Broth w/SPS + CO

✔

Blood Culture Bottles Fluid

Thioglycollate Medium w/SPS + CO

✔

Blood Culture BottlesThiol

Broth w/CO

✔

Blood Culture BottlesThiol

Broth w/SPS + CO

✔

Blood Culture Bottles

Tryptic Soy Broth w/CO

✔

Blood Culture Bottles Tryptic

Soy Broth w/SPS + CO

SEPTIC-CHEK

™

TSB w/ SPS + CO

†

✔

Brain Heart Infusion Agar

†

✔

Brilliant Green Agar

†

✔

Coagulase Plasma (Rabbit)/ Coagulase

Plasma, Rabbit

†

✔

SFP Agar Base/TSN Agar

†

✔

Simmons Citrate Agar

†

✔

SPS Agar

†

✔

Sterile Swab/Aerobic Collection & Trans. System w/o Agar

†

✔

Triple Sugar Iron Agar

†

✔

Tryptic Soy Agar/TRYPTICASE

™

Soy Agar

†

✔

Tryptic Soy Broth/TRYPTICASE

™

Soy Broth

†

✔

Tryptose Phosphate Broth

†

✔

Veterinary Testing - Products and Applications

PRODUCTS

Salmonella/Shigella

Streptococcus

Staphylococcus

Mycological Media

Blood Culture

APPLICATIONS

Susceptibility Testing

Vibrio

Oxidation-Fermentation

(O-F) Test

Section VI Veterinary Testing - Products and Applications

PRODUCTS

APPLICATIONS

Collection/Transport

Bacterial

Collection/Transport, Viral

Clostridium

Francisella

Leptospira

Pseudomonas

General Purpose Bacterial

Culture Media

Brucella

Gram Negative Enteric

Bacteria (General)

†

Available from Difco & Becton Dickinson Microbiology Systems.

840 The Difco Manual

Coagulase Plasma EDTA (Rabbit)/ Coagulase

Plasma, Rabbit w/EDTA

†

✔

Columbia CNA Agar

†

✔

Corn Meal Agar

†

✔

Decarboxylase Medium Base ✔

Dispens-O-Disc Susceptilbility Disks: Apramycin 15 mcg

✔

Ceftiofur 30 mcg ✔

Enrofloxacin 5 mcg ✔

Tilmicosin 15 mcg ✔

DNase Test Agar

†

✔

DNase Test Agar w/ Methyl Green/DNase

Test Agar w/Toluidine Blue

†

✔

DTM Agar ✔

Lysine Iron Agar

†

✔

Mannitol Salt Agar

†

✔

MIO Medium ✔

MR-VP Medium/MR-VP Broth

†

✔

Mueller Hinton Medium/Mueller Hinton II Agar

†

✔

Mycological Agar/MYCOPHIl

™

Agar

†

✔

OF Basal Medium

†

✔

Phenol Red Broth Base

†

✔

Phenylethanol Agar/Phenylethyl Alcohol Agar

†

✔✔

Purple Agar Base ✔

Purple Broth Base

†

✔

Sabouraud Dextrose Agar

†

✔

Sabouraud Dextrose Broth ✔

Selenite Broth/Selenite F Broth ✔

Selenite Cystine Broth

†

✔

SS Agar/Salmonella Shigella Agar

†

✔

Staph Latex Test/STAPHYLOSLIDE

™

Test Kit

†

✔

Strep Grouping Kit

†

✔

Tetrathionate Broth Base

†

✔

TCBS Agar

†

✔

Urea Agar Base

†

✔

Vibrio Cholerae Antisera ✔

VJ Agar (Vogel-Johnson Medium)

†

✔

XLD Agar

†

✔

XLT4 Agar Base and XLT4 Supplement ✔

Veterinary Testing - Products and Applications

Veterinary Testing-Products and Applications Section VI

PRODUCTS

Salmonella/Shigella

Streptococcus

Staphylococcus

Mycological Media

Blood Culture

APPLICATIONS

Susceptibility Testing

Vibrio

Oxidation-Fermentation

(O-F) Test

†

Available from Difco & Becton Dickinson Microbiology Systems.

The Difco Manual 841

A-1 Medium/A-1 Broth

†

✔

Azide Dextrose Broth

†

✔

BAGG Broth ✔

Baird-Parker Agar Base w/

EY Tellurite Enrichment/Egg Yolk Tellurite Solution

†

✔

Bile Esculin Agar

†

✔

Bile Esculin Azide Agar/ENTEROCOCCOSEL

™

Agar

†

✔

Bismuth Sulfite Agar

†

✔

Brilliant Green Agar

†

✔

Brilliant Green Agar Modified

(Edel-Kempelmacher) ✔

Brilliant Green Bile 2%/Brilliant Green Bile Broth 2%

†

✔

m Brilliant Green Broth ✔

Desoxycholate Lactose Agar

†

✔

m E Agar/M-E Agar Base

†

✔

EC Medium/EC Broth

†

✔

EC Medium with MUG/EC Broth w/ MUG

†

✔

m Endo Agar LES/m Endo Agar LES

†

✔

m Endo Broth MF

/m Endo Broth

†

✔

m Enterococcus Agar/m Enterococcus Agar

†

✔

Esculin Iron Agar ✔

EVA Broth/Ethyl Violet Azide Broth

†

✔

m FC Agar/ m FC Agar

†

✔

m FC Basal Medium ✔

m FC Broth Base/m FC Broth

†

✔

m HPC Agar/m HPC Agar Base

†

✔

Lactose Peptone Broth ✔

Lauryl Tryptose Broth/Lauryl Sulfate Broth

†

✔

Lauryl Tryptose Broth with MUG/

Lauryl Sulfate Broth w/ MUG

†

✔

Levine EMB Agar/Levine Eosin Methylene Blue Agar

†

✔

Minerals Modified Glutamate Broth ✔

Muller Kauffmann Tetrathionate

Broth Base ✔

Water/Wastewater Testing - Products and Applications

Section VI Water/Wastewater Testing-Products and Applications

PRODUCTS

Standard Plate Count

Total Coliforms

Fecal Coliforms /

E. coli

APPLICATIONS

Fecal Streptococcus

Staphylococcus

Salmonella

Stressed Organisms

†

Available from Difco & Becton Dickinson Microbiology Systems.