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822 The Difco Manual
Food and Beverage Testing - Products and Applications
Lactobacilli MRS Agar/MRS Agar
†
✔
Lactobacilli MRS Broth/MRS Broth
†
✔
Lactose Broth
†
✔
Lauryl Tryptose Broth/Lauryl Sulfate Broth
†
✔
Lauryl Tryptose Broth with MUG/Lauryl Sulfate Broth w/MUG
†
✔
Letheen Agar
†
✔
Letheen Broth/Letheen Broth AOAC
†
✔
Levine EMB Agar/Levine Eosin Methylene Blue Agar
†
✔
Listeria Enrichment Broth
†
✔
Listeria O Antisera ✔
Liver Veal Agar
†
✔
Lysine Medium ✔ ✔
MYP Agar ✔
M Broth
†
✔
MacConkey Agar
†
✔✔
MacConkey Sorbitol Agar/MacConkey II Agar w/Sorbitol
†
✔
Malt Agar
†
✔
Malt Extract Agar
†
✔
Malt Extract Broth
†
✔
Mannitol Salt Agar
†
✔
McBride Listeria Agar
†
✔
McClung Toabe Agar Base
†
✔
Microbial Content Test Agar/
TRYPTICASE
™
Soy Agar w/Lec. & Polysorbate 80
†
✔
Minerals Modified Glutamate Agar ✔
Modified Listeria Enrichment Broth ✔
Modified Oxford Antimicrobic Supplement ✔
Modified EC Medium w/Indicator and
Novobiocin Antimicrobic Supplement ✔
Motility Test Medium
†
✔
Moxalactam Antimicrobic Supplement
†
✔
Muller Kauffmann Tetrathionate Broth Base ✔
Mycological Agar/MYCOPHIL
™
Agar
†
✔
Neutralizing Buffer
†
✔
OGYE Agar Base w/Antimicrobic Vial Oxytetracycline
✔
Orange Serum Agar
†
✔
Orange Serum Broth Concentrate 10X ✔
Oxford Antimicrobic Supplement ✔
Oxford Medium Base/Oxford Agar Base Modified
†
✔
PALCAM Medium Base with
PALCAM Antimicrobic Supplement ✔
Plate Count Agar/Standard Methods Agar
†
✔
Potato Dextrose Agar
†
✔
Potato Dextrose Broth
†
✔
Rappaport-Vassiliadis (MSRV) Medium
Semisolid Modification ✔
Food and Beverage Testing - Products and Applications Section VI
PRODUCTS
APPLICATIONS
Bacillus
Beverage Analysis
Campylobacter
Analysis
Clostridium
Coliform Analysis
Environmental
Lactobacillus
Yeast & Mold Analysis
Yersinia
sp.
Vibrio
Salmonella/Shigella
Staphylococcus
Total Plate Count
Listeria
Analysis
†
Available from Difco & Becton Dickinson Microbiology Systems.
The Difco Manual 823
Food and Beverage Testing - Products and Applications
Rappaport-Vassiliadis R10 Broth ✔
Raka-Ray No. 3 Broth ✔✔
Raka-Ray No. 3 Medium ✔✔
Reinforced Clostridial Agar
†
✔
Rogosa SL Agar
†
✔
Rogosa SL Broth
†
✔
Rose Bengal Agar Base ✔
Rose Bengal Antimicrobic Supplement C/
Chloramphenicol Selective
†
✔
SFP Agar Base/TSN Agar
†
✔
SS Agar/Salmonella Shigella Agar
†
✔
Sabouraud Dextrose Agar
†
✔
Sabouraud Dextrose Broth ✔
Salmonella H Antiserum Poly a-z
†
✔
Salmonella O Antisera
†
✔
Selenite Broth/Selenite F Broth
†
✔
Selenite Cystine Broth
†
✔
Special Yeast and Mold Medium ✔
m Staphylococcus Broth ✔
Staphylococcus Medium 110/Staphylococcus Agar 110
†
✔
Staph Latex Test/STAPHYLOSLIDE
™
Test
†
✔
Sulfite Agar ✔
TCBS Agar
†
✔
TT Broth Base, Hajna/Tetrathionate H
†
✔
Tetrathionate Broth Base
†
✔
Tomato Juice Agar
†
✔
Tryptic Soy Broth/TRYPTICASE
™
Soy Broth
†
✔
Tryptone Water ✔
UBA Medium/Universal Beer Agar
†
✔
UVM Modified Listeria Enrichment Broth
†
✔
Universal Preenrichment Broth ✔✔
Vibrio Cholerae Antisera ✔
Violet Red Bile Agar
†
✔
Violet Red Bile Agar w/MUG
†
✔
Violet Red Bile Glucose Agar ✔
WL Differential Medium/WL Differential Agar
†
✔
WL Nutrient Broth ✔
WL Nutrient Medium
†
✔
Wort Agar
†
✔ ✔
XLD Agar
†
✔
XLT4 Agar Base and XLT4 Supplement ✔
YM Agar ✔
YM Broth ✔
Yeast Extract Glucose Chloramphenicol Agar ✔
Yersinia Selective Agar Base/CIN Agar Base
†
✔
Yersinia Antimicrobic Supplement CN/CN Inhibitor
†
✔
Section VI Food and Beverage Testing - Products and Applications
PRODUCTS
APPLICATIONS
Bacillus
Beverage Analysis
Campylobacter
Analysis
Clostridium
Coliform Analysis
Environmental
Lactobacillus
Yeast & Mold Analysis
Yersinia
sp.
Vibrio
Salmonella/Shigella
sp.
Staphylococcus
Total Plate Count
Listeria
Analysis
†
Available from Difco & Becton Dickinson Microbiology Systems.
824 The Difco Manual
McFarland Standard Preparation
Molecular Genetics - Media and Ingredients
1. Prepare the tubes by mixing 1% sulfuric acid with 1% barium chloride according to the table.
2. Make sure the tubes are uniform in size and made of chemically resistant glass.
3. Plug the tubes with rubber stoppers and carefully seal with paraffin. Store the tubes upright.
4. To estimate bacterial cell density, compare the bacterial suspension with the standards.
5. The above set is used to determine bacterial density in saline suspension. To estimate bacterial density
in broth, make the set by dissolving the sulfuric acid and barium chloride in sterile broth.
REFERENCE IN: Gradwohl's Clinical Laboratory Methods and Diagnosis. In. A.C. Sonnenwirth and
L. Jarett (ed.). C.V. Mosby Company, 1980 p. 1363.
McFarland Standard Preparation & Molecular Genetics - Media and Ingredients Section VI
†
Available from Difco & Becton Dickinson Microbiology Systems.
LB Agar, Lennox/LB Agar
(Lennox LAgar)
†
LB Agar, Miller (Luria-Bertani)/
Luria Agar
†
LB Broth, Lennox/LB Broth
†
LB Broth, Miller/Luria Broth
†
Luria Agar Base, Miller
Luria Broth Base, Miller
Casein Digest/Casein Digest
Peptone
†
NZCYM Broth
†
NZM Broth
NZYM Broth/NZY Broth
†
LB MEDIA NZ MEDIA AND INGREDIENTS GENERAL MEDIA AND INGREDIENTS
M9CA Medium
M9 Minimal Salts, 5x/
M9 Minimal Salt
†
Minimal Agar Davis
Minimal Broth Davis w/o Dextrose
SOB Medium
Terrific Broth
2xYT
YPD Agar/YEPD Agar
†
YPD Broth/YEPD Broth
†
Yeast Nitrogen Base
†
Yeast Nitrogen Base w/o Amino Acids
†
Yeast Nitrogen Base w/o Amino
Acids & Ammonium Sulfate
1 9.9 0.1 300 3
2 9.8 0.2 600 7
3 9.7 0.3 900 10
4 9.6 0.4 1200 12
5 9.5 0.5 1500 15
6 9.4 0.6 1800 –
7 9.3 0.7 2100 20
8 9.2 0.8 2400 –
9 9.1 0.9 2700 –
10 9.0 1.0 3000 30
"MCFARLAND" SULFURIC ACID, 1% BARIUM CHLORIDE, 1% CORRESPONDING INTERNATIONAL
AQUEOUS SOLUTION AQUEOUS SOLUTION DENSITY OF UNITS (IU)
ML ML BACTERIA -10
6
OF OPACITY
The Difco Manual 825
Mycobacteria
Middlebrook 7H9 Broth/Middlebrook 7H9 Broth Base
†
Middlebrook 7H10 Agar/Middlebrook & Cohn 7H10
Agar Base
†
Middlebrook ADC Enrichment
†
Middlebrook OADC Enrichment
Middlebrook OADC Enrichment
w/WR 1339
Mycobacteria 7H11 Agar/Seven H11 Agar Base
†
Petragnani Medium
ATS Medium
Dubos Albumin Broth
Dubos Broth Base
Dubos Medium Albumin
Dubos Oleic Agar Base
Dubos Oleic Albumin Complex/
Oleic Albumin Complex
†
ESP Myco
MYCOBACTERIA
ESP Myco GS
ESP Myco PVNA
Lowenstein Medium Base/
Lowenstein Jensen Medium Base
†
Lowenstein Medium Gruft
Lowenstein Medium, Jensen
Lowenstein Medium, Jensen Deeps
Lowenstein Medium w/5% NaCl
Section VI Mycobacteria
†
Available from Difco & Becton Dickinson Microbiology Systems.
826 The Difco Manual
Mycology
MEDIA
Vitamin Assay
General Use
Blood Agar Base
†
✔
Brain Heart Infusion
†
✔
Brain Heart Infusion Agar
†
✔
Brain Heart Infusion w/PAB
†
✔
Corn Meal Agar
†
✔
Fluid Sabouraud Medium ✔
Malt Agar
†
✔
Malt Extract Agar
†
✔
Malt Extract Broth
†
✔
Mycological Agar/MYCOPHIL
™
Agar
†
✔
Mycological Agar w/Low pH/MYCOPHIL
™
w/ Low pH
†
✔
Oatmeal Agar ✔
Pagano Levin Base and TTC ✔
Potato Dextrose Agar
†
✔
Potato Dextrose Broth
†
✔
Rice Extract Agar
†
✔
SABHI Agar Base/SABHI Agar
†
✔
Sabouraud Agar Modified/
Sabouraud Dextrose Agar Emmons
†
✔
Sabouraud Dextrose Agar
†
✔
Sabouraud Dextrose Broth ✔
Sabouraud Maltose Agar
†
✔
Special Yeast and Mold Medium ✔
YM Agar ✔
YM Broth ✔
NON-INHIBITORY MEDIA FOR FUNGI
CLINICAL REAGENTS FOR DETECTION
OF
CANDIDA ALBICANS
BiGGY Agar
†
Candida Isolation Agar
Candida Albicans Antiserum
MEDIA FOR CLASSIFICATION OF YEASTS
Yeast Carbon Base
Yeast Morphology Agar
Yeast Nitrogen Base
†
Yeast Nitrogen Base w/o Amino Acids
†
Yeast Nitrogen Base w/o Amino Acids and
Ammonium Sulfate
Candida
and/or
Dermatophytes
Mycology Section VI
†
Available from Difco & Becton Dickinson Microbiology Systems.
The Difco Manual 827
Reagents for Direct Microscopic Detection of Fungi
Selective and/or Differential Media for Fungi
Brain Heart CC Agar
†
Cooke Rose Bengal Agar and Antimicrobic Vial A
DRBC Agar
HC Agar Base
†
Mycobiotic Agar/Mycosel
™
Agar
†
Mycological Agar w/Low pH/Mycophil
™
Agar w/Low pH
†
Rose Bengal Agar Base and Rose Bengal Antimicrobic
Supplement C
Yeast Extract Glucose Chloramphenicol Agar
BiGGY Agar
†
Candida BCG Agar Base
Candida Isolation Agar
DTM Agar
Littman Oxgall Agar
Trichophyton Agars 1-7
GENERAL USE
CANDIDA
AND/OR DERMATOPHYTES
Mycology
Section VI Mycology
Gram Stain Sets and Reagents
†
General screening
3-Step™ Gram Stain Sets and Reagents General screening
SpotTest™ Calcofluor White Reagent/Calcofluor White Droppers
†
Screening of cultures for presence of fungi
A nonspecific fluorochrome stain which allows the rapid
examination of clinical specimens for the presence of fungi
under an FA microscope
SpotTest India Ink/India Ink Droppers
†
Screening CSF for Cryptococcus neoformans
For use in the direct microscopic examination of clinical material
Negative staining
for the presence of encapsulated yeast cells
SpotTest KOH 10%/Potassium Hydroxide 10% Droppers
†
Used in wet mounts of clinical specimens to
A 10% solution of potassium hydroxide examine for the presence of fungi
SpotTest Lactophenol Cotton Blue Stain/ Teased mount method
Lactophenol Cotton Blue Droppers
†
Slide culture method
For use in the direct mounting and staining of yeast and molds
PRODUCT USES
†
Available from Difco & Becton Dickinson Microbiology Systems.
828 The Difco Manual
AK Amikacin 32,24,16-1 32-4 32-4 32,24,16-4 –––––– –––––– –––––– ––––––
A/C Amoxicillin/ –––––– –––––– 16-4/8-2 –––––– 16-0.5/8-0.25 16-0.25/8-0.12 –––––– ––––––
Clavulanic Acid
AM Ampicillin 16-2 16-2 16-8 16-8 16-0.12 –––––– 8-0.12 8-0.12
A/S Ampicillin/ 16-2/8-1 16-8/8-4 16-8/8-4 16-8/8-4 –––––– –––––– 16-2/8-1 16-2/8-1
Sulbactam
AZT Aztreonam 16-4 16-8 16-8 16-8 –––––– –––––– –––––– ––––––
CB Carbenicillin –––––– –––––– –––––– –––––– 256-128 256-128 –––––– ––––––
CCL Cefaclor –––––– –––––– –––––– –––––– 16-1 16-1 –––––– ––––––
CFZ Cefazolin 16-2 16-2 16-8 16-8 –––––– –––––– 16-2 16-8
FIX Cefixime 2-1 2-1 –––––– 2-1 2-0.25 –––––– 2-0.5 2-1
CPZ Cefoperazone 32-4 32-16 32-16 32-16 –––––– –––––– –––––– ––––––
CTX Cefotaxime 32-8 –––––– 32-8 32-8 –––––– 16-0.12 32-4 32-8
CTN Cefotetan 32-8 32-8 32-16 32-16 –––––– –––––– –––––– ––––––
CX Cefoxitin 16-2 16-4 16-8 16-8 –––––– –––––– –––––– ––––––
TAZ Ceftazidime 16-2 16-4 16-4 16-8 –––––– –––––– –––––– ––––––
CTZ Ceftizoxime 32-4 32-8 –––––– 32-8 –––––– –––––– –––––– ––––––
FRX Ceftriaxone 32-4 32-8 32-8 32-8 –––––– 8-0.25 32-4 32-8
CFX Cefuroxime 16-2 16-2 16-8 16-8 16-0.5 4-0.25 16-4 16-8
CF Cephalothin –––––– –––––– 16-8 16-8 16-1 16-8 –––––– ––––––
C Chloramphenicol 16-2 –––––– 16-8 16-8 16-0.5 8-1 16-4 16-4
CIP Ciprofloxacin 2-0.25 2-0.25 2-1 2-1 2-0.06 –––––– 2-0.25 2-0.25
CLM Clarithromycin –––––– –––––– –––––– –––––– 4-0.25 4-0.12 4-0.5 4-0.5
CD Clindamycin –––––– –––––– –––––– –––––– 2-0.25 2-0.06 2-0.25 2-0.25
EN Enoxacin –––––– –––––– –––––– 4-2 –––––– –––––– –––––– ––––––
E Erythromycin –––––– –––––– –––––– –––––– 4-0.25 4-0.25 4-0.5 4-0.5
GM Gentamicin 8,6,4-0.25 8,6,4-0.5 8,6,4-0.5 8,6,4-1 –––––– –––––– 500,8,6,4-0.25 500,8,6,4-1
IMI Imipenem –––––– –––––– 8-4 –––––– –––––– 8-0.06
LOM Lomefloxacin 4-2 4-2 –––––– 4-2 4-2 –––––– 4-2 4-2
MZ Mezlocillin 64-8 64-8 64-16 –––––– –––––– –––––– –––––– ––––––
NET Netilmicin 16-8 –––––– –––––– –––––– –––––– –––––– –––––– ––––––
FD Nitrofurantoin 64-32 64-32 64-32 64-32 64-32 64-32 –––––– 64-32
NOR Norfloxacin 8-4 ______ 8-4 8-4 8-4 8-2 –––––– ––––––
OFX Ofloxacin 4-0.5 4-0.5 4-2 4-2 4-0.12 8-1 4-0.25 4-1
OX Oxacillin –––––– –––––– –––––– –––––– 6,4-0.5 –––––– 6.4-0.5 6,4-1
P Penicillin –––––– –––––– –––––– –––––– 8-0.03 2-0.03 8-0.03 8-0.03
PIP Piperacillin 64-8 ______ 64-16 64-8 –––––– –––––– –––––– ––––––
P/T Piperacillin/ 64-8/4 64-8/4 64-16/4 64-16/4 –––––– –––––– 16-2/4 16-4/4
Tazobactam
RI Rifampin –––––– –––––– –––––– –––––– 2-1 4-0.5 2-1 2-1
STR Streptomycin –––––– –––––– –––––– –––––– –––––– 1000 1000
SFX Sulfisoxasole –––––– –––––– –––––– –––––– 256 –––––– –––––– ––––––
TE Tetracycline –––––– –––––– 8-4 8-4 8-0.5 8-1 8-2 ––––––
TC Ticarcillin –––––– –––––– –––––– 64-8 –––––– –––––– –––––– ––––––
T/C Ticarcillin/ –––––– –––––– 64-16/2 –––––– –––––– 64-16/2 –––––– ––––––
Clavulanic Acid
TO Tobramycin 8,6,4-0.25 8,6,4,-0.5 8,6,4,-0.5 8,6,4-1 –––––– –––––– 8,6,4-0.25 8,6,4-1
T/S Trimethoprim/ 2-1/38-19 2-1/38-19 2-38 2/38 2-0.5/38-9.5 2-0.5/38-9.5 2-0.5/38-9.5 2/38
Sulfamethoxazole
VA Vancomycin –––––– –––––– –––––– –––––– 16-4 4-0.5 16-1 16-2
Biochemical Substrates
30* 30* 30* 18**
Pasco
®
Panel Contents - Selection Guide
ANTIMICROBIAL AGENT MIC MIC/ID MIC/ID GRAM- BREAKPOINT/ID MIC MIC MIC MIC/ID
(MCG/ML) GRAM-NEG. GRAM-NEG. NEG. SPECIAL GRAM-NEG. ORAL SUPP. GRAM-POS. GRAM-POS.
Pasco
®
Panel Contents - Selection Guide Section VI
* Gram-Negative Panels
** Gram-Positive Panels
The Difco Manual 829
Sulfate
Sulfur
Tin
Zinc
Calcium
Chloride
Cobalt
Copper
Iron
Lead
Magnesium
Manganese
Phosphate
Potassium
Sodium
Glycine
Histidine
Isoleucine
Leucine
Lysine
Methionine
Alanine
Arginine
Aspartic Acid
Phenylalanine
Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
Beef Extract 2.54 1.39 1.67 0.18 6.01 4.14 4.94 0.53 1.00 1.45 0.30 <0.01 2.16 0.90 0.67 0.05 1.99 0.86
Beef Extract, Dessicated 8.96 5.66 4.30 0.17 12.55 16.25 2.50 1.45 3.63 3.27 1.08 2.00 9.58 2.10 1.42 0.32 1.03 2.62
Casamino Acids 3.26 2.20 4.76 0.16 15.30 1.31 1.66 3.34 5.47 5.71 1.28 2.11 6.17 2.19 2.41 <0.01 0.47 4.30
Casamino Acids, Technical 1.64 1.77 3.42 0.34 10.97 1.09 1.31 0.13 2.54 2.14 1.19 5.23 4.44 2.64 1.99 0.01 1.33 3.24
Casein Digest 2.92 3.02 6.75 0.17 23.10 1.93 2.52 4.66 8.29 7.70 2.66 4.27 11.04 5.55 4.33 1.16 2.54 6.51
Casitone 3.01 3.76 6.61 0.02 20.03 1.97 2.17 4.16 8.74 13.62 1.71 4.02 8.57 4.82 3.74 0.14 2.09 4.06
Neopeptone 4.03 4.14 6.19 0.26 13.22 7.02 <0.01 0.36 3.65 5.16 2.00 8.67 6.73 4.22 3.69 0.96 4.21 4.96
Bacto Peptone 8.67 6.76 5.60 0.20 10.21 15.59 0.58 1.45 3.01 3.42 1.19 1.81 8.80 2.87 1.81 0.36 0.64 2.35
Proteose Peptone 6.50 5.12 7.28 0.87 11.95 9.68 2.01 3.04 5.66 5.33 1.97 2.86 5.93 3.49 3.14 0.60 2.35 3.76
Proteose Peptone No. 2 6.08 5.47 7.45 0.40 10.57 10.84 <0.01 1.00 3.57 5.22 1.51 7.94 5.31 4.64 3.90 0.94 1.92 4.73
Proteose Peptone No. 3 5.99 5.49 6.92 1.12 12.38 9.26 1.74 2.65 5.70 5.02 1.86 2.72 4.94 3.65 3.32 0.59 1.96 3.62
Soytone 2.46 3.82 7.27 1.45 12.76 2.51 1.24 2.37 4.03 3.45 0.86 2.46 2.92 2.87 2.17 0.47 1.93 2.65
TC Lactalbumin Hydrolysate 3.70 2.67 7.13 0.55 16.30 2.02 1.83 1.18 5.43 4.11 2.11 10.81 6.62 4.62 4.76 1.75 1.22 5.82
TC Yeastolate 4.84 2.99 5.58 0.45 10.53 4.02 <0.01 0.56 3.23 3.82 0.96 5.59 2.59 2.81 2.80 0.79 1.21 3.80
Tryptone 2.86 3.03 6.11 0.42 17.05 1.75 2.02 4.40 7.11 6.70 2.57 3.71 7.45 4.29 3.58 0.71 1.42 5.00
Tryptose 4.45 4.65 6.34 0.44 13.92 2.84 <0.01 0.34 3.67 4.64 1.92 7.52 6.33 4.09 3.55 0.62 2.21 1.93
Yeast Extract 5.36 3.02 6.69 0.74 14.20 3.25 1.20 3.23 4.69 5.15 1.05 2.53 2.60 2.84 2.95 1.36 1.20 3.79
Cystine
Glutamic Acid
AMINO ACIDS - %
Beef Extract 0.068 1.284 <0.001 <0.001 <0.001 <0.001 0.239 <0.001 5.458 5.477 2.315 0.629 0.707 <0.001 <0.001 0.2
Beef Extract, Dessicated 0.018 1.576 <0.001 0.001 0.011 <0.001 0.022 <0.001 0.345 1.994 2.774 0.829 0.661 <0.001 0.002 <0.1
Casamino Acids <0.001 7.400 <0.001 <0.001 <0.001 <0.001 0.002 <0.001 3.325 0.410 8.710 0.045 0.420 <0.001 <0.001
Casamino Acids, Technical 0.019 21.212 <0.001 <0.001 <0.001 <0.001 0.008 <0.001 1.358 0.273 13.721 0.167 0.424 <0.001 0.002 3.4
Casein Digest 0.019 0.059 <0.001 <0.001 0.002 <0.001 0.018 <0.001 2.670 0.078 2.937 0.167 0.621 <0.001 0.002 4.2
Casitone 0.010 0.110 <0.001 <0.001 0.003 <0.001 0.019 <0.001 2.604 0.162 3.073 0.339 0.676 <0.001 0.004 0.2
Neopeptone 0.012 0.344 <0.001 <0.001 <0.001 <0.001 0.006 <0.001 2.209 0.149 2.057 0.340 0.657 <0.001 <0.001 0.8
Bacto Peptone 0.008 1.086 <0.001 <0.001 0.004 <0.001 0.007 <0.001 0.445 0.203 1.759 0.244 0.410 <0.001 0.001 6.9
Proteose Peptone 0.021 4.510 <0.001 <0.001 0.002 <0.001 0.027 <0.001 0.872 0.685 3.677 0.162 0.812 <0.001 0.002 <0.1
Proteose Peptone No. 2 0.024 3.644 <0.001 <0.001 <0.001 <0.001 0.024 <0.001 1.674 0.815 3.956 0.232 0.698 <0.001 0.003 1.3
Proteose Peptone No. 3 0.023 3.581 <0.001 <0.001 0.002 <0.001 0.027 <0.001 1.447 0.982 3.815 0.232 0.975 <0.001 0.007 1.4
Soytone 0.055 0.165 <0.001 <0.001 0.008 <0.001 0.161 <0.001 0.820 2.220 3.404 2.334 1.660 <0.001 0.001 24.0
TC Lactalbumin Hydrolysate 0.095 <0.010 <0.001 <0.001 0.002 <0.001 0.026 <0.001 1.309 0.932 1.357 0.379 0.750 <0.001 0.002 9.2
TC Yeastolate 0.002 0.299 <0.001 <0.001 <0.001 <0.001 0.025 <0.001 2.633 5.085 0.819 0.488 0.528 <0.001 0.007 10.3
Tryptone 0.013 0.186 <0.001 <0.001 <0.001 <0.001 0.017 <0.001 2.669 0.229 2.631 0.241 0.740 <0.001 0.003 7.7
Tryptose 0.001 1.886 <0.001 <0.001 0.002 <0.001 0.022 <0.001 2.144 0.679 3.410 0.308 0.737 <0.001 0.005 7.1
Yeast Extract 0.013 0.380 <0.001 <0.001 <0.001 <0.001 0.075 <0.001 3.270 3.195 1.490 0.091 0.634 <0.001 0.011 17.5
PRODUCT
INORGANICS - %
CARBOHYDRATE
Total (%)
Peptones & Hydrolysates Selection Guide
Typical Analyses
Section VI Peptones & Hydrolysates Selection Guide
PRODUCT
830 The Difco Manual
Ash (%)
Clarity 1% Soln (NTU)
Filterability (g/cm
2
)
Coliform
Salmonella
Spore Count
Standard Plate Count
Thermophile Count
Total Nitrogen (%)
Amino Nitrogen
AN/TN (%)
Beef Extract 24.1 116.8 0.1 77.2* 5.4 neg neg 299 117 33 11.2 3.8 33.8
Beef Extract, Dessicated 10.2 1.7 0.6 2.5 6.9 neg neg 585 690 28 14.0 2.2 15.7
Casamino Acids 24.4 0.5 2.9 4.5 6.4 neg neg 390 950 25 10.5 8.8 83.8
Casamino Acids, Technical 38.3 0.3 2.6 4.5 6.7 neg neg 2375 2250 <50 8.1 6.4 79.0
Casein Digest 6.4 0.4 2.6 4.7 7.2 neg neg 235 250 178 13.4 7.2 53.9
Casitone 7.0 0.6 1.7 3.7 7.2 neg neg 300 1850 100 13.3 4.7 35.3
Neopeptone 7.0 1.2 0.3 3.2 7.4 neg neg 175 400 75 13.7 3.3 23.8
Bacto Peptone 4.4 0.5 0.5 3.0 7.0 neg neg 90 273 13 15.5 3.1 20.0
Proteose Peptone 11.1 1.4 0.9 3.1 7.2 neg neg 393 443 73 14.0 2.9 20.7
Proteose Peptone No. 2 12.7 1.5 0.6 3.5 7.2 neg neg 75 1450 <50 12.6 5.0 39.7
Proteose Peptone No. 3 11.4 2.2 0.5 4.0 7.2 neg neg 890 915 25 13.2 3.5 26.5
Soytone 12.0 1.0 1.2 4.6 7.2 neg neg 10 38 <3 9.4 3.1 33.0
TC Lactalbumin Hydrolysate 7.2 0.4 7.3 4.6 7.1 neg neg <50 300 <50 13.0 6.3 48.3
TC Yeastolate 13.0 1.4 4.5 3.6 6.9 neg neg 175 175 <50 10.8 6.5 59.8
Tryptone 6.8 0.5 1.3 3.7 7.2 neg neg 73 870 8 13.0 5.2 40.0
Tryptose 9.7 0.8 2.3 3.2 7.4 neg neg 875 825 100 13.4 4.4 32.5
Yeast Extract 11.2 1.5 2.7 3.1 6.7 neg neg 9 60 <5 10.9 6.0 55.0
Nicotinic Acid
PABA
Pantothenic Acid
Pyridoxine
Riboflavin
Thiamine
Beef Extract 0.1 1171.5 0.5 3.3 4113.2 774.7 20.0 91.0 7.3 0.4 <0.1 1093.4
Beef Extract, Dessicated 0.1 1300.0 <0.1 0.6 2100.0 138.1 40.5 8.7 2.8 <0.1 <0.1 111.3
Casamino Acids <0.1 160.0 <0.1 <0.1 <100.0 <20.0 <5.0 <0.1 <0.1 1.8 1.2 <30.0
Casamino Acids, Technical <0.1 <50.0 <0.1 <0.1 <38.0 <0.1 9.8 <0.6 <0.1 0.2 0.2 <14.0
Casein Digest 0.1 <40.0 <0.1 1.0 490.0 14.1 6.1 6.7 0.4 <0.1 1.5 8297.0
Casitone 0.2 550.0 <0.1 0.8 980.0 20.3 15.9 7.7 1.3 0.4 <0.1 342.9
Neopeptone 0.2 3100.0 <0.1 0.4 3600.0 52.2 2.9 16.0 2.3 1.3 <0.1 <14.0
Bacto Peptone 0.2 2000.0 <0.1 0.3 2400.0 21.9 <0.5 5.9 1.7 3.9 <0.1 413.0
Proteose Peptone 0.1 2300.0 <0.1 0.4 5000.0 79.9 4.2 20.0 1.1 <0.1 1.2 99.7
Proteose Peptone No. 2 0.3 4500.0 <0.1 0.5 4700.0 157.1 1.2 47.0 4.0 6.4 1.6 1319.0
Proteose Peptone No. 3 0.4 3700.0 <0.1 0.3 8900.0 124.2 <0.5 20.0 1.3 6.8 0.1 659.6
Soytone 0.2 2200.0 <0.1 3.0 2100.0 19.1 9.0 13.0 11.0 <0.1 1.2 113.2
TC Lactalbumin Hydrolysate <0.1 280.0 <0.1 0.2 360.0 <0.1 11.3 4.3 1.0 8.5 0.5 <14.0
TC Yeastolate 6.7 3400.0 <0.1 25.2 1900.0 945.0 96.6 300.0 77.5 21.8 54.3 2975.0
Tryptone 0.1 350.0 <0.1 0.3 1400.0 97.8 3.7 5.3 0.6 <0.1 0.4 93.4
Tryptose 0.2 2700.0 <0.1 0.4 5400.0 47.4 11.4 16.0 1.4 4.3 0.1 769.0
Yeast Extract 3.3 300.0 <0.1 1.5 1400.0 597.9 763.0 273.7 43.2 116.5 529.9 217.5
Thymidine
Biotin
Choline
Cyanocobalamin
Folic Acid
Inositol
PRODUCT
Loss on Drying (%)
pH, 1% Soln
PHYSICAL CHARACTERISTICS
PRODUCT
VITAMINS - µg/g
BIOLOGICAL TESTING - CFU/g NITROGEN CONTENT
*Represents Total Solids value rather than loss on drying.
Peptones & Hydrolysates Selection Guide
Typical Analyses
Peptones & Hydrolysates Selection Guide Section VI
The Difco Manual 831
Pharmaceutical Testing - Products and Applications
Vitamin/Amino Acid Assay
APPLICATIONS
PRODUCTS
Sterility Testing
Antibiotic Assay
Agar Medium No. F ✔
Antibiotic Medium 1
†
✔
Antibiotic Medium 2/Base Agar, Penicillin Assay
†
✔
Antibiotic Medium 3
†
✔
Antibiotic Medium 4/Yeast Beef Agar
†
✔
Antibiotic Medium 5/
Streptomycin Assay Agar w/YE
†
✔
Antibiotic Medium 8/Base Agr w/Low pH
†
✔
Antibiotic Medium 9/Polymixin Base Agar
†
✔
Antibiotic Medium 10/Polymixin Seed Agar
†
✔
Antibiotic Medium 11/Neomycin Assay Agar
†
✔
Antibiotic Medium 12 ✔
Antibiotic Medium 19/Nystatin Assay Agar
†
✔
APT Agar
†
✔
APT Broth
†
✔
Aseptic Commissioning Medium ✔
B12 Assay Medium USP ✔
B12 Culture Agar USP ✔
B12 Inoculum Broth USP ✔
Biotin Assay Medium ✔
Cystine Assay Medium ✔
Fluid Thioglycollate Medium
†
✔
Fluid Thioglycollate Medium w/Beef Extract
†
✔
Fluid Thioglycollate Medium w/K Agar ✔
Folic Acid Assay Medium ✔
Folic Acid Casei Medium ✔
Folic AOAC Medium ✔
Folic Buffer A, Dried ✔
Inositol Assay Medium ✔
Lactobacilli Agar AOAC ✔
Lactobacilli Broth AOAC ✔
Lysine Assay Medium ✔
Methionine Assay Medium ✔
Section VI Pharmaceutical Testing - Products and Applications
†
Available from Difco & Becton Dickinson Microbiology Systems.