Ian M. Rosenberg Birkh?user Boston; 2nd edition (September 29,
2004)
Великолепный сборник методов по выделению, очистке и изучения структуры белков. В книге описаны методы изучения пострансляционных модификаций белков, хроматографии, методы получения рекомбинантных белков, работы с мембранными белками, электрофоретического разделения белков. Несомненно окажется полезной для любой лаборатории, сфера исследований которой связана с изучением белков.
Из английской аннотации к книге:
New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry, and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how," this new edition is a complete resource for the benchside.
Contents
Chapter 1 An Overview of This Manual
Chapter 2 Protein Structure
Chapter 3 Tracking the Target Protein
Chapter 4 Electrophoretic Techniques
Chapter 5 Getting Started with Protein Purification
Chapter 6 Membrane Proteins
Chapter 7 Transfer and Detection of Proteins on Membrane Supports
Chapter 8 Identification of the Target Protein
Chapter 9 Identifying and Analyzing Posttranslational Modifications
Chapter 10 Chromatography
Chapter 11 Recombinant Protein Techniques
Великолепный сборник методов по выделению, очистке и изучения структуры белков. В книге описаны методы изучения пострансляционных модификаций белков, хроматографии, методы получения рекомбинантных белков, работы с мембранными белками, электрофоретического разделения белков. Несомненно окажется полезной для любой лаборатории, сфера исследований которой связана с изучением белков.
Из английской аннотации к книге:
New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry, and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how," this new edition is a complete resource for the benchside.
Contents
Chapter 1 An Overview of This Manual
Chapter 2 Protein Structure
Chapter 3 Tracking the Target Protein
Chapter 4 Electrophoretic Techniques
Chapter 5 Getting Started with Protein Purification
Chapter 6 Membrane Proteins
Chapter 7 Transfer and Detection of Proteins on Membrane Supports
Chapter 8 Identification of the Target Protein
Chapter 9 Identifying and Analyzing Posttranslational Modifications
Chapter 10 Chromatography
Chapter 11 Recombinant Protein Techniques