Springer-Verlag Berlin, 2008, 496 pages
The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis. For the first time, a team of recognized inteational experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field. This first of two volumes covers basic aspects and various techniques such as steady-state and time-resolved fluorometry, polarization techniques, and fluorescent chemical sensors.
The aim of this book is to provide a unique overview on the current state of instrumentation and application employed for steady state and time-resolved fluorometry and fluorescence polarization measurement as well as fluorescence techniques and materials used for fluorescent VIII Preface
chemical sensing thereby highlighting the present state of quality assurance and the need for future standards. Method-inherent advantages, limitations, and sources of uncertainties are addressed, often within the context of typical and upcoming applications. The ultimate goal is tomake users of fluorescence techniques more aware of necessary steps to improve the overall reliability and comparability of fluorescence data to encourage the further broadening of fluorescence applications.
The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis. For the first time, a team of recognized inteational experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field. This first of two volumes covers basic aspects and various techniques such as steady-state and time-resolved fluorometry, polarization techniques, and fluorescent chemical sensors.
The aim of this book is to provide a unique overview on the current state of instrumentation and application employed for steady state and time-resolved fluorometry and fluorescence polarization measurement as well as fluorescence techniques and materials used for fluorescent VIII Preface
chemical sensing thereby highlighting the present state of quality assurance and the need for future standards. Method-inherent advantages, limitations, and sources of uncertainties are addressed, often within the context of typical and upcoming applications. The ultimate goal is tomake users of fluorescence techniques more aware of necessary steps to improve the overall reliability and comparability of fluorescence data to encourage the further broadening of fluorescence applications.