Vlak J.M., de Gooijer C.D., Tramper J., Miltenburger H.G. (Eds.) Insect Cell Cultures: Fundamental and Applied Aspects
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INSECT CELL CULTURES: FUNDAMENTAL AND APPLIED ASPECTS
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Insect Cell Cultures:
Fundamental and Applied Aspects
Edited by
J.M. VLAK
Dept. of Virology, Wageningen Agricultural University,
The Netherlands
C.D. de GOOIJER, J. TRAMPER
Dept. of Food Science, Wageningen Agricultural University,
The Netherlands
and
H.G. MILTENBURGER
Dept. of Zoology, Technical University, Darmstadt, Germany
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eBook ISBN: 0-306-46850-6
Print ISBN: 0-7923-3403-5
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Print ©1996 Kluwer Academic Publishers
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Table of Contents
Preface
Development and characterization of insect cell lines
Dwight E. Lynn
New approaches to insect tissue culture
Danica Baines
Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene
Ann Marie Fallon
Insect cell physiology
Ravinder Bhatia, Gary Jesionowski, Jerome Ferrance & Mohammad M. Ataai
Insect cell cultivation: growth and kinetics
Georg Schmid
Medium design for insect cell culture
Ernst- Jürgen Schlaeger
Baculovirus–insect cell interactions
Gary W. Blissard
Replication patterns and cytopathology of cells infected with baculoviruses
Greg V. Williams & Peter Faulkner
Construction of baculovirus recombinants
Paul A. Kitts
Passage effect of virus infection in insect cells
Peter J. Krell
Post-translational modifications in insect cells
Hans-Dieter Klenk
Productivity of insect cells for recombinant proteins
James E. Maruniak
Chaperone and foldase coexpression in the baculovirus-insect cell expression system
Michael J. Betenbaugh, Eric Ailor, Erik Whiteley, Paul Hinderliter & Tsu-An Hsu
Shear sensitivity of insect cells
Jeffrey J. Chalmers
Insect cell bioreactors
Spiros N. Agathos
Perfusion bioreactors for the production of recombinant proteins in insect cells
Volker Jäger
Immobilization of insect cells
Jian-Yong Wu & Mattheus F. A. Goosen
Modelling baculovirus infection of insect cells in culture
John Francis Power & Lars Keld Nielsen
Scale up aspects of sparged insect-cell bioreactors
J. Tramper, J.M. Vlak & C.D. de Gooijer
Oxygen gradients in small and big sparged insect-cell bioreactors
J. Tramper, J.M. Vlak & C. D. de Gooijer
Downstream processing of insect cell cultures
Alain R. Bernard, Manjula Lusti-Narasimhan, Kathryn M. Radford, Richard S. Hale, Eric Sebille &
Pierre Graber
vii
3–11
13–22
23–32
33–41
43–55
57–70
73–93
95–110
111–123
125–137
139–144
145–148
149–160
163–171
173–189
191–198
199–208
209–219
221–229
231–238
239–257
Parvovirus diagnostics and vaccine production in insect cells
José Ignacio Casal
Classical swine fever virus diagnostics and vaccine production in insect cells
Marcel M. Hulst & Rob J.M. Moormann
Production of multidomain complement glycoproteins in insect cells
Péter Závodzky & Sándor Cseh
Economics of baculovirus – insect cell production systems
David J. Rhodes
Safety aspects of insect cell culture
G. Stacey & R. Possee
Regulatory issues in the use of insect-cell culture
Frank L.J. van Lier
List of Contributors
Keyword index
261–270
271–277
279–288
291–297
299–303
305–309
Cytotechnology
20:
vii, 1996. vii
Special Issue
Preface
The remarkable growth and application of insect cell culture research during the past decade has been driven by
several biotechnological advances. Of greatest significance was the development in the 1980’s of the application of
recombinant DNA technology to express cloned genes in insect host cells. The emergence of the baculovirus-insect
cell system as a versatile gene expression tool resulted from intensive and elegant studies on the molecular biology
of baculoviruses, and the establishment of new insect cell culture systems which supported high levels of protein
expression. The baculovirus expression system has not only become an important tool for research, but is now
accepted as an important technology for the commercialization of products for use in agriculture and human health.
Insect cell cultures fundamental and applied aspects provides a comprehensive review of the major advances
in insect cell culture research. The book is divided into five sections which systematically approach the topic,
from the basic principles of cell culture technology through the concomitant economic and regulatory issues that
are important aspects of the commercialization of bio-based products. All chapters in this volume are written by
the foremost authorities in the field who have contributed original research in insect cell and molecular biology,
chemical engineering, and baculovirology. The first part is devoted to an in-depth review of basic insect cell culture
and cell biology with chapters on new advances in transgenic cells and novel media formulations. Part two presents
an exceptionally lucid survey of the molecular biology of baculoviruses and their development as expression vectors.
These introductory virus chapters are followed by specialized topics on the expression of recombinant proteins
and their posttranslational modification. Part three, devoted to review chapters on the application of bioengineering
principles for the scale-up of insect cell culture, also discusses the differences between invertebrate and vertebrate
cell culture and the impact of cell bioreactor design, shear force concerns, nutrient and oxygen requirements that are
specific for insect cells. Parts four and five detail examples of specific commercial products produced in insect cell
systems. The book concludes with an exhaustive consideration of the economic and regulatory issues that have to be
considered by industry and government agencies. The topics discussed are interrelated through a focus on the insect
cell culture-baculovirus system and the prominent role it plays in basic biological research and biotechnology.
The book benefits from clear and precise writing throughout. All chapters are well documented with figures,
illustrations, and comprehensive bibliographies. This authoritative volume will serve as a standard information
resource on insect cell culture. The book will become the benchmark reference manual for students and researchers
in academia and government laboratories requiring information on how insect cell systems are being applied in
basic research and biotechnology applications. In particular this treatise will be an invaluable source of knowledge
to industrial scientists who are applying the baculovirus-cell culture systems for commercial purposes.
Robert R. Granados
Ithaca, New York
March, 1996
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PART I
INSECT CELL LINES