Corley R.B. A Guide to Methods in the Biomedical Sciences
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A GUIDE TO METHODS IN THE BIOMEDICAL SCIENCES
Quantitative “real-time” PCR, 56
Quaternary protein structure, 21
quaternary structure, 3
radiation chimeras, 113
radioimmunoassay, 75, 79
Radioimmunoassay (RIA), 78, 79
RAG-deficient blastocyst complementation,
112
recombinant DNA technology, 15, 25, 29, 30,
39, 40, 43,
51
Reducing and non-reducing SDS-PAGE, 8
Reporter assays, 61, 62
Reporter plasmids, 46
Representation difference analysis (RDA), 59
representative cDNA libraries, 38, 50
restriction digestion, 27
Restriction enzymes and restriction fragments,
29, 30
restriction fragment, 29, 31, 64, 67
Restriction fragment length polymorphism
(RFLP), 30
restriction mapping, 30
retroviruses, 37, 38, 49
Reverse transcriptase (RNA-dependent DNA
polymerase), 37, 38
Reverse transcription PCR (RT-PCR), 55
reversed phase HPLC (RP-HPLC), 16
RFLP, 30, 67
Ribonuclease (RNase) protection assay
(RPA), 36, 37
ricin, 16
74
RNA interference (RNAi), 66
rotary shadowing, 97
RT-PCR, 38, 55
S1 analysis, 37
sandwich ELISA, 79
Sanger, Dansyl chloride, 17
Scanning electron microcope (SEM), 97, 98
Scientific American, 53
Screening for differentially expressed genes
(DEGs), 57
SDS-PAGE, 7–12, 21, 22, 64, 65
secondary and tertiary protein structure, 19
secondary antibodies, 13
secondary antibody, 22, 79, 81
secondary structure, 3, 19
second-step reagent, 76
Sedimentation equilibrium, 4
Sedimentation velocity, 5
selectable marker, 40, 48, 108, 109, 111
Semiquantitative PCR, 55
Sequencing vectors, 46
Serial analysis of gene expression (SAGE),
60
Short tandem repeat (STR), 67
Silencing gene expression, 65, 66
silver stained, 9
Silver staining, 11
simple sequence length polymorphisms,
106
Single nucleotide polymorphisms (SNP), 30,
31
site directed mutagenesis, 25, 51, 65
Site Directed Mutagenesis, 51–52
small interfering RNAs (siRNA), 66
Somatic cell genetics, 72
Southern blotting, 13, 30, 31, 36, 57
Southwestern blot, 65
Specialized vectors, 44
Speed congenic strains of mice, 105,
106
stains, 10
stare and compare, 9, 59
Streptavidin, 76
streptolysin-O (SLO), 91
stringency, 32
Subcellular fractionation, 5
Subtractive hybridization, 58
Sucrose density gradients, 5
Supershift assay, 63
Surface plasmon resonance, 85
SYBR Green, 56
Taq polymerase, 54, 56
probe, 56
Targeted transgenes, 108–112
targeting vector, 108–109
tertiary structure, 3
tester, 59
tetraparental mice, 113
Total internal reflection (TIR) fluorescence
microscopy, 93, 94
transduction, 24, 43
Transformation, 42
Transgenic mice, 106–107
Transient or stable expression of eukaryotic
expression vectors, 48
transmission electron microscope, 88
Transmission electron microscope (TEM),
95
TUNEL assay, 85
Two-dimensional (2D) gel electrophoresis, 9
Two-photon excitation microscopy, 93
ultracentrifuge, 2, 4, 5
Ultrathin sectioning and plastic embedding,
96
Index
125
UV cross-linking and immunoprecipitation,
63
vaccinia virus, 49
Variations on TEM, 97
vectors, 30, 33, 34, 39–50, 108, 111
velocity sedimentation, 5
viral plaques, 43, 44
Viral vectors, 48, 49
Western blot analysis (Immunoblotting), 13
western blotting, 9, 11, 13, 16, 21, 22,
64,
85
X-ray crystallography, 19, 20
x-ray diffraction, 2, 19, 20
Y chromosome analysis, 67, 68
yeast artificial chromosomes (YAC), 45
Yeast two-hybrid screen, 65
yellow fluorescent protein (YFP), 47
YFP,
47, 91, 94
Zinc staining, 12
z-stack, 89, 92
44, 46, 61
44