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A GUIDE TO METHODS IN THE BIOMEDICAL SCIENCES
Differential display, 59
Differential interference contrast (DIC), 89–90
Differential screening, 57–58
direct immunofluorescence, 81
DNA chips, 60
DNA content, cell cycle analysis, and
apoptosis, 83
DNAfootprinting, 64
DNA microarrays, 60
DNA sequencing, 32–35
Dot blotting and slot blotting, 31
driver, 59
Edman degradation, 17
electroblotting, 13, 31
electron microscope, 87, 88, 95, 97
Electron Microscopy (EM), 95–98
Electrophoresis, 6
Electrophoretic mobility shift assay (EMSA),
62–63
electroporation, 42, 48, 109, 111
embryonic stem cells, 65, 108
enhanced chemiluminescence (ECL), 13, 76
Enzyme-linked antibodies, 76–77
Enzyme-linked immunosorbent assay (ELISA),
79
Enzyme-linked immunospot (ELISPOT)
assays, 80
epitopes, 69, 78
Eukaryotic expression vectors, 47–48
Expressed Sequence Tags (EST), 50
Expression vectors, 47
FACS, 80–83
Far western blot, 22
Fast Protein Liquid Chromatography (FPLC),
16
FLAG tag, 47
Flow cytometry and fluorescence activated cell
sorting (FACS), 80–83
Floxed\genes in knockout technology, 111
FLP-FRT system, 111–112
fluorescence activated cell sorting, 77, 80
Fluorescence and laser confocal microarray
microscopy, 90–95
fluorescence microscopy, 90, 93
Fluorescence recovery after photobleaching
(FRAP), 94
Fluorescence resonance energy transfer
(FRET), 24
Fluorescent antibodies, 77
Fluorescent molecules, 90–91
Forensics and DNA technology, 66
FPLC, 16
fraction collectors, 14
Freeze-fracture and deep etching, 97
FRET (fluorescence resonance energy
transfer), 24
Gel filtration (or “size exclusion”)
chromatography, 14
Gels and gradients for separation of
macromolecules, 4
Gels and gradients for separation of nucleic
acids, 27
gene chips, 60
Gene expression studies, 84, 85
gene profiling, 61
gene silencing, 65, 66
genomic libraries, 30, 43, 49, 50
Genomic library, 50
green fluorescent protein (GFP), 46, 47, 91
GST (glutathione-S-transferase) tag, 47
HAT medium, 72
74
High-performance liquid chromatography
(HPLC), 16
His
tag,
47
homologous recombination, 65, 108, 109
horseradish peroxidase (HRP), 13, 76
HPLC, 16
Human Genome Project, 46, 58
Human monoclonal antibodies, 74
hybridized, 32, 36, 52, 58, 60
hybridomas, 73
hydrophobic interaction chromatography 15
Identification of proteins in gels by staining,
11,
12
Identification of proteins in gels using
metabolic labeling, 12
immunoassay, 78
immunoblotting, 13
immunocytochemistry, 85
immunofluorescence, 76, 81, 82, 92
Immunogold electron microscopy, 96
Immunohistochemistry (IH or IHC), 85
immunoprecipitate, 21, 64
Immunoprecipitation, 78
immunostaining, 85
In silico cloning, 50
in vitro transcription-translation assays, 78
Inbred strains of mice, 102–103
indirect immunofluorescence, 81
insert, 28, 33, 42, 44, 46
inserts, 41, 43–45, 49
lodinated antibodies, 75