Humana, 2010. - 322 p. - In recent years, the critical role of
microRNAs has been revealed within the biology of cells that
constitute the immune system. In MicroRNAs and the Immune System:
Methods and Protocols, expert researchers explore the latest
techniques for studying miRNA expression, including the most
up-to-date data on splinted ligation and qRT-PCR assays, as well as
high-throughput profiling through cloning, deep sequencing, and
microarrays. Chapters outline methods to study miRNA functions in
various cell types from a single cell type level to entire model
organisms, and present studies of miRNAs in the context of viruses
and the immune response. Tools are also provided to help navigate
bioinformatics databases on miRNAs and their targets. Composed in
the highly successful Methods in Molecular Biology™ series format,
each chapter contains a brief introduction, step-by-step methods, a
list of necessary materials, and a Notes section which shares tips
on troubleshooting and avoiding known pitfalls. Contemporary and
innovative, MicroRNAs and the Immune System: Methods and Protocols
is an essential handbook for immunologists, biochemists, and
molecular biologists.
Monticelli S. (Ed.). MicroRNAs and the Immune System: Methods and Protocols
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- размер 31.3 МБ
- добавлен 28 января 2012 г.
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